Targeting detoxification genes by phloem-mediated RNAi: A new approach for controlling phloem-feeding insect pests

Eakteiman, Galit; Moses-Koch, Rita; Moshitzky, Pnina; Mestre-Rincon, Natividad; Vassão, Daniel Giddings; Luck, Katrin; Sertchook, Rotem; Malka, Osnat; Morin, Shai

doi:10.1016/j.ibmb.2018.05.008

Cited by 46 publications

(39 citation statements)

References 92 publications

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“…In total, three biological replicates, with three technical replicates were conducted, and the qPCR was performed as previously described (Kang et al, 2017b). However, in this study, we used a 2 −ΔCt method to evaluate the expression of selected genes (Eakteiman et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

et al. 2018

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The integrated pest management (IPM) strategy was developed and used in combination with pesticides and beneficial biological control agents. To further develop IPM efficiency, it is important to evaluate the side effects of pesticides on biological control agents. Aphidius gifuensis is one of the most important aphid natural enemies and has been successfully used to control Myzys persicae and other aphid species. Imidacloprid (IMD) is a popular pesticide used worldwide and is highly toxic to non-target arthropods. Here, we investigated the short-term sublethal toxicity of IMD in Aphidius gifuensis and its impact on the biological performance and gene expression of this parasitoid. We found that sublethal IMD doses had a significant negative effect on the life history traits of female A. gifuensis, including shortening the lifespan and lowering parasitic capacity. Moreover, exposure to sublethal IMD also adversely affected the response of A. gifuensis to aphid-infested plant volatiles. Based on the transcriptome analysis, we found that the exposure to sublethal IMD doses significantly affected expression of genes involved in the central nervous system, energy metabolism, olfactory, and detoxification system of A. gifuensis. RT-qPCR also revealed that short term expose to sublethal IMD doses significantly induced the gene expression of genes related to the central nervous system (nAChRa7, nAChRa9, TbH, OAR1, NFR, TYR, and DAR1), olfactory system (OR28 and IR8a1), and detoxification system (CYP49p3, CYP6a2, and POD), while it suppressed the expression of genes involved in the central nervous system (nAChRa4 and nAChRb1), olfactory system (Orco1, IR8a2, and GR1), and detoxification system (GST2). Furthermore, exposure to sublethal doses of IMD also significantly increased the activities of CarEs and POD, whereas we observed no influence on the activities of CAT, GST, and SOD. Our results indicate that sublethal IMD doses might adversely affect the biological performance of A. gifuensis by altering gene expression related to the function of olfactory, nervous, energy metabolism, and detoxification systems. Thus, how the use of pesticides directly affect insect population should be considered when used in conjunction with natural pest parasitoids in IPM strategies.

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Section: Methodsmentioning

confidence: 99%

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

et al. 2018

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“…If true, the present considerations must be expanded to include strategies that minimize ecological impacts and maximize long-term efficacy. This sentiment echoes increasing calls for more holistic approaches to RNAi-mediated pest management [ 58 ]. For the remainder of this review, we focus on three key considerations that have been underrepresented in studies of RNAi in pest management: ecological impact, evolution of resistance, and choice of functional pathway.…”

Section: Rnai As a Pest Management Strategy: Need For Greater Specmentioning

confidence: 99%

“…Moreover, successful implementation of this new technology remains hindered by familiar pest management issues, including cost efficiency and ecological impacts, that ultimately limit the success of RNAi as a long-term solution. Whiteflies are remarkably well studied among insect pests, and many aspects of successful RNAi implementation in B. tabaci have been worked out [ 29 , 30 , 31 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. According to overarching consensus, the formula for effective RNAi pest management of B. tabaci is to (1) select a target gene that is crucial for survival, (2) use a plant-mediated delivery method, and (3) prevent dsRNA degradation.…”

Section: Rnai As a Pest Management Strategy: Need For Greater Specmentioning

confidence: 99%

“…Categories of sublethal target pathways include those involved in gametogenesis, immunity, movement, mating, and appetite stimulation. Targeting genes from pathways involved in the detoxification of plant defenses has already shown to be successful at reducing, but not eradicating, B. tabaci populations [ 58 ]. Table 1 provides examples of potential targets.…”

Section: Selecting Suitable Targets Based On Pathway Involvementmentioning

confidence: 99%

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Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly Bemisia tabaci

Shelby

Moss

Andreason

et al. 2020

Insects

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The whitefly Bemisia tabaci is a globally important pest that is difficult to control through insecticides, transgenic crops, and natural enemies. Post-transcriptional gene silencing through RNA interference (RNAi) has shown potential as a pest management strategy against B. tabaci. While genomic data and other resources are available to create highly effective customizable pest management strategies with RNAi, current applications do not capitalize on species-specific biology. This lack of specificity has the potential to have substantial ecological impacts. Here, we discuss both short- and long-term considerations for sustainable RNAi pest management strategies for B. tabaci, focusing on the need for species specificity incorporating both life history and population genetic considerations. We provide a conceptual framework for selecting sublethal target genes based on their involvement in physiological pathways, which has the greatest potential to ameliorate unintended negative consequences. We suggest that these considerations allow an integrated pest management approach, with fewer negative ecological impacts and reduced likelihood of the evolution of resistant populations.

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“…20,21 The BtGSTs5 in the whitefly Bemisia tabaci can accept hydrolyzed glucosinolates and produce their corresponding detoxified conjugates. 22 In contrast, other insects can prevent release of isothiocyanates by redirecting the glucosinolate hydrolysis to less or nontoxic products. 5,23 These specialized strategies are often thought to be characteristic of specialist feeders.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

Lei

Chen

et al. 2020

Pest Management Science

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BACKGROUND: Overexpressing CIRCADIAN CLOCK ASSOCIATED1 in Arabidopsis thaliana (CCA1-ox) increases indole glucosinolate production and resistance to green peach aphid (Myzus persicae). Little is known of how aphids respond to this group of plant defense compounds or of the underlying molecular mechanism. Results: Aphids reared on CCA1-ox for over 40 generations (namely the CCA population) became less susceptible to CCA1-ox than aphids maintained on the wild-type Col-0 (namely the COL population). This elevated tolerance was transgenerational as it remained for at least eight generations after the CCA population was transferred to Col-0. Intriguingly, transcriptome analysis indicated that all differential cytochrome P450 monooxygenase genes (MpCYPs), primarily MpCYP4s, MpCYP380s and MpCYP6s, were more highly expressed in the CCA population. Application of a P450 inhibitor to the CCA population resulted in decreased aphid reproduction on CCA1-ox, which was not observed if aphids were reared on Col-0. When indole glucosinolate biosynthesis in CCA1-ox was blocked using virus-induced gene silencing, the effect of the P450 inhibitor on the CCA population was attenuated, affirming the essential role played by MpCYPs in counteracting the defense mechanism in CCA1-ox that is low or absent in Col-0. Furthermore, we used host-induced gene silencing to identify MpCYP380C6 and MpCYP380C9 that specifically facilitated the CCA population to cope with CCA1-mediated plant defense. Expression profiles revealed their possible contribution to the transgenerational tolerance observed in aphids. Conclusion: MpCYP380C6 and MpCYP380C9 in aphids play a crucial role in mitigating indole glucosinolate-mediated plant defense, and this effect is transgenerational.

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Targeting detoxification genes by phloem-mediated RNAi: A new approach for controlling phloem-feeding insect pests

Cited by 46 publications

References 92 publications

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly Bemisia tabaci

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

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