Microfluidic paper-based analytical devices and micro total analysis systems are relatively new group of analytical tools, capable of analyzing complex biochemical samples containing macromolecules, proteins, nucleic acids, toxins, cells or pathogens. Within one analytical run, fluidic manipulations like transportation, sorting, mixing or separation are available. Recently, microfluidic devices are a subject of extensive research, mostly for fast and non-expensive biochemical analysis but also for screening of medical samples and forensic diagnostics. They are used for neurotransmitter detection, cancer diagnosis and treatment, cell and tissue culture growth and amplification, drug discovery and determination, detection and identification of microorganisms. This review summarizes development history, basic fabrication methods, applications and also future development trends for production of such devices.

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In this study, we determined the concentrations of total mercury (Hg) in samples of liver, kidney and skeletal muscle of 27 red foxes Vulpes vulpes (L., 1758) from north-western Poland, and examined the morphometric characteristics of the collected specimens. The analysis also included the relationship between Hg concentration and the fox size, and the suitability of individual organs as bioindicators in indirect evaluation of environmental mercury contamination. Determination of Hg concentration was performed by atomic absorption spectroscopy. In the analysed samples, the Hg concentration was low and the maximum value did not exceed 0.85 mgHg/kg dry weight (dw). There were no significant differences in Hg concentrations in the analysed material between males and females or between immature and adult groups. The median concentrations of Hg in the liver, kidney and skeletal muscle were 0.22, 0.11 and 0.05 mgHg/kg dw, respectively. The correlation coefficients were significant between the concentrations of mercury in the liver, kidney and skeletal muscle (positive) and between the kidney Hg concentration and kidney mass (negative). Taking into account our results and findings of other authors, it may be argued that the red fox exhibits a measurable response to mercury environmental pollution and meets the requirements of a bioindicator.

The aim of this study was to determine the concentration of mercury (Hg) in liver (L), kidney (K), breast muscle [BM (musculus pectoralis major)], breast feathers (BF), and stomach contents (SC) of mallard (Anas platyrhynchos L. 1758). Among the edible parts of mallard, the greatest concentrations of Hg were observed in K and L, although they did not exceed 1.5 mg/kg dry weight (dw). Average concentrations in K, L, and BM were 0.27, 0.25, and 0.13 mg/kg dw, respectively. Significant correlations were observed between Hg concentrations in BM and K and in BM and L (rs = 0.92) as well as between Hg concentrations in these tissues and BF. In addition, we found significant correlations between Hg concentrations in SC and BM (rs = 0.72) and in L and K (rs = 0.55). In conclusion, mallard exhibits a measurable response to environmental Hg pollution and meets the requirements of a bioindicator.

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