Microfluidic Paper-Based Analytical Devices (μPADs) and Micro Total Analysis Systems (μTAS): Development, Applications and Future Trends

Lisowski, Piotr; Zarzycki, Paweł K.

doi:10.1007/s10337-013-2413-y

Cited by 208 publications

(127 citation statements)

References 178 publications

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“…Among the various microfluidic devices, microfluidic paper-based analytical devices (μPADs) have became a subject of research activities attributing to their advantages of cost effectiveness, disposability, simple fabrication, low sample volume required, and wettability (which helps eliminate external flow control systems) and ease of storage and delivery without damage (Cheng et al, 2010;Liana et al, 2012;Lisowski and Zarzycki 2013;Meredith et al, 2016). Unlike lateral flow assays which are difficult to obtain multiplex and qualitative results, μPADs can simultaneously detect multiple analytes from extracted samples and receive qualitative results via a scanner or smartphone (Parolo and Merkoçi 2013).…”

Section: Microfluidic Paper-based Analytical Devicementioning

confidence: 99%

A mini-review on functional nucleic acids-based heavy metal ion detection

Zhan

Wang

et al. 2016

Biosensors and Bioelectronics

143

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Section: Microfluidic Paper-based Analytical Devicementioning

confidence: 99%

A mini-review on functional nucleic acids-based heavy metal ion detection

Zhan

Wang

et al. 2016

Biosensors and Bioelectronics

143

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“…The microsystems in medicine and biology often belong to one of the four important areas of application: diagnostics, drug delivery, minimally invasive surgery, or neural prosthetics and tissue engineering [4]. Concerning the diagnostic area there are three categories, most of the test systems are based on: paper-based analytical devices, lab-on-chip, and micro total analysis system (μTAS) [5].…”

Section: Introductionmentioning

confidence: 99%

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Tröger¹,

Niemann²,

Gärtig³

et al. 2015

J Nanomed Nanotechnol

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Nucleic acid amplification technologies (NAATs) offer the most sensitive tests in the clinical laboratory. These techniques are used as a powerful tool for screening and diagnosis of infectious diseases. Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results.However, current molecular diagnostic approaches generally need manual analysis by qualified and experienced personal which is a highly complex, time-consuming and labor-intensive task. Thus, the demand for simpler, miniaturized systems and assays for pathogen detection is steadily increasing. Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis.In this article, we review several isothermal amplification methods and their implementation in microsystems in relation to quantification of nucleic acids. miniaturized systems can be diverse. The majority of publications are focussed on clinical diagnostics including foodborne organisms for environmental monitoring [10][11][12]. In case of an infectious disease, cultivation and phenotypic characterization are still the standard methods of microbiologists which need days up to weeks to obtain a diagnostic result. Hence, scientists started to make use of nucleic acid amplification methods to accelerate the analysis. The most widespread and well known technique for this purpose is the polymerase chain reaction (PCR) [10,13], which exploits the activity of the DNA polymerase. The method is based on a thermal cycling process consisting of repeated cycles of heating and cooling steps allowing for DNA melting, sequence specific primer binding and enzymatic amplification of the target nucleic acid. The quantitative assessment of target copies is possible by using a real-time PCR approach, where a concentration dependent signal (e.g. fluorescence) increases over time [14]. The latter enables the user to easily assess the pathogen load of patient samples [15][16][17][18]. Since the first example of a PCR on a miniaturized system in 1994 [19,20], numerous devices were presented, which integrate not just the amplification, but also sample preparation and detection steps [9,[21][22][23][24][25][26][27][28]. Just a few of those microsystems have reached the market so far, but some have shown a fascinating potential to replace the classical laboratory-oriented state-of-the art [29][30][31][32][33]. setups has been shown to obtain a similar result [34]. A smaller heat capacity allows for rapid changes in temperature beneficial for the PCR time as well as a higher parallelism of multiple genetic samples [34].However, a major drawback of the integration of PCR to microsystems is the necessity of sophisticated instrumentation. The reaction requires a thermal cycling instrumentation, space and considerable expertise [35]. Therefore, isothermal reactions for the ta...

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“…As for NCC, one can observe a smooth and compact morphology (Figure 3b) and also a needle-like structure in the cross-section inset. The low porosity, roughness in the order of nanometers, and high compactness of the cellulose fibers give these paper substrates a high contact angle (~95°C, accessed through 1 µL of deionized water droplet), which translates in a significant hydrophobicity that is beneficial to microfluidic devices [79], self-cleaning and to prevent the negative effects that the absorption of water can have on electronics devices.…”

Section: Light Papermentioning

confidence: 99%

Optoelectronics and Bio Devices on Paper Powered by Solar Cells

Vicente¹,

Araújo²,

Gaspar³

et al. 2017

Nanostructured Solar Cells

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The employment of printing techniques as cost-effective methods to fabricate low cost, flexible, disposable and sustainable solar cells is intimately dependent on the substrate properties and the adequate electronic devices to be powered by them. Among such devices, there is currently a growing interest in the development of user-oriented and multipurpose systems for intelligent packaging or on-site medical diagnostics, which would greatly benefit from printable solar cells as their energy source for autonomous operation.This chapter first describes and analyzes different types of cellulose-based substrates for flexible and cost effective optoelectronic and bio devices to be powered by printed solar cells. Cellulose is one of the most promising platforms for green recyclable electronics and it is fully compatible with large-scale printing techniques, although some critical requirements must be addressed. Paper substrates exist in many forms. From common office paper, to packaging cardboard used in the food industry, or nanoscale engineered cellulose (e.g. bacterial cellulose). However, it is the structure and content of paper that determines its end use. Secondly, proof-of-concept of optoelectronic and bio devices produced by inkjet printing are described and show the usefulness of solar cells as a power source or as a chemical reaction initiator for sensors.

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Microfluidic Paper-Based Analytical Devices (μPADs) and Micro Total Analysis Systems (μTAS): Development, Applications and Future Trends

Cited by 208 publications

References 178 publications

A mini-review on functional nucleic acids-based heavy metal ion detection

A mini-review on functional nucleic acids-based heavy metal ion detection

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Optoelectronics and Bio Devices on Paper Powered by Solar Cells

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