Dammarane-type saponins, gypenosides VN1-VN7 (1-7), were isolated from the total saponin extract of Gynostemma pentaphyllum aerial parts, with their structures elucidated on the basis of spectroscopic and chemical methods. These compounds showed moderate cytotoxic activity against four human cancer cell lines, A549 (lung), HT-29 (colon), MCF-7 (breast), and SK-OV-3 (ovary), with IC(50) values ranging from 19.6+/-1.1 to 43.1+/-1.0 microM. Regarding the HL-60 (acute promyelocytic leukemia) cell line, compounds 1, 5, and 6 showed weakly active with IC(50) values of 62.8+/-1.9, 72.6+/-3.6, and 82.4+/-3.2 nM, respectively, while 2, 3, 4, and 7 were less active with IC(50) values>100 microM.
Computer vision has been currently a new trend in developing new tools for automatic real-time quality control process in food drying. During drying process, the size and shape of mango slides are critically changed. These changes usually determine the sensorial value of products, as well as which drying conditions would be needed to obtain the highest quality of dried products. In this study, we report on the development of a computer vision tool, requiring a normal digital camera installed, to evaluate the changes in size and shape of mango slides during the drying process. The technique is expected to replace the observation with human eyes to evaluate the changes of food products during the drying process, which might not be able to provide reliable and consistent judgements all the time. Image of drying mango slide is taken by a digital camera, then the feature extraction is implemented. The area of mango slice is determined by the area ratio via the pixel number counting and the comparison to an original sample with predefined reference size. The shrinkage deformation is evaluated by elliptical fitting to develop the automated utility. The utility is built on MATLAB platform. The variations in size and shape of the mango slices during a convective drying process with different processing conditions are examined and acquired by the built software which achieves real-time performance on the personal laptop.
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