No abstract

The anti-tumour protein alpha-sarcin causes fusion of bilayers of phospholipid vesicles at neutral pH. This is demonstrated by measuring the decrease in the efficiency of the fluorescence energy transfer between N-(7-nitro-2-1,3-benzoxadiazol-4-yl)-dimyristoylphosphatidylethano lamine (NDB-PE) (donor) and N-(lissamine rhodamine B sulphonyl)-diacylphosphatidylethanolamine (Rh-PE) (acceptor) incorporated in dimyristoylphosphatidylcholine (DMPG) vesicles. The effect of alpha-sarcin is a maximum at 0.15 M ionic strength and is abolished at basic pH. alpha-Sarcin promotes fusion between 1,6-diphenylhexa-1,3,5-triene (DPH)-labelled DMPG and dipalmitoyl-PG (DPPG) vesicles, resulting in a single thermotropic transition for the population of fused phospholipid vesicles. Bilayers composed of DMPC and DMPG, at different molar ratios in the range 1:1 to 1:10 PC/PG, are also fused by alpha-sarcin. Freeze-fracture electron micrographs corroborate the occurrence of fusion induced by the protein. alpha-Sarcin also modifies the permeability of the bilayers, causing the leakage of calcein in dye-trapped PG vesicles. All of the observed effects reach saturation at a 50:1 phospholipid/protein molar ratio, which is coincident with the binding stoichiometry previously described.

No abstract

This paper presents the findings from a small-scale experiment investigating the presentation of a synchronous remote electronic examination. It discusses the students' experiences of taking such an examination. The study confirms that the majority of participants found the experience at least as good as a conventional written examination. In addition, typing answers does not prevent students from producing answers in the time available. However, the pressure of time continues to be a major cause of anxiety for students. The paper discusses technical issues, particularly those related to the loss of communications during the 3-hour duration of the exam. Although software processes were available to save and restore students' answers throughout the examination, problems still occurred and more robust software is required.

No abstract

This study confirms that CPS is an independent predictor of all-cause morbidity and mortality in older trauma patients. However, CPS was not independently associated with need for discharge to a facility. Prospective multicenter studies are needed to evaluate the use of CPS as a predictive and interventional tool, with special focus on correlations between specific pre-existing conditions, pharmacologic interactions, and morbidity/mortality patterns.

This is the first of a series of papers describing how the Open University, a large distance education institution, has embraced the Internet as a medium for supporting its students, tutors, academics, and administrators throughout the education process. This paper reports on a holistic approach to integrating technology into the teaching process which addresses how to provide necessary functions in effective formssome traditional, some new. It describes the development and structure of actual systems, which incorporate electronic student registration; electronic assignment submission, marking, and recording; electronic tutorials and other interaction; and electronic examinations. It describes and summarizes some of the findings of the evaluation of those systems, including student, academic, and administrator feedback. Finally, this paper reflects on the benefits of an integrated holistic approach in exploiting the Internet's potential to support distance learning on a massive scale. Subsequent papers in this series will address specific areas in detail: the student experience, the role of the tutor in an electronic environment, and the institutional perspective.

Background : Pharmacy is an information intensive profession and a pharmacist has to be proficient in health care information management. The advance in information technology and the use of computers to access, retrieve and analyse this information is increasingly important. Yet little is known about pharmacists' computer literacy or information technology skills. Objectives : To gain a better understanding of pharmacists' basic computer skills and their ability to use standard software. Methods : Self-administered postal survey to 747 registered pharmacists residing in two counties of England. Results : A total of 386 (52%) pharmacists responded after two mailings. Most responding pharmacists used computers at work and at home. They believed their basic computing skills to be high, although acknowledged that they were skill deficient in using certain software packages. Internet use at work was low (43%) as too was awareness and use of online medical databases; this was reflected in online medical database training being identified as of greatest need. Conclusions : Pharmacists were confident in operating computer systems but identified a training need for particular software applications. Of concern is the limited knowledge they possessed and access they had on medical databases.