In order to reveal the friction properties and improve the wear resistance of silicon nitride ceramic materials, a calculation model of static friction coefficient of silicon nitride ceramic is established. The influence law of contact surface roughness on friction coefficient under different contact load and friction speed is analyzed. The test and the results verification are carried out by using Friction Wear Testing Machine. Then the dry friction process of silicon nitride ceramic is simulated based on UDEC, and the wear failure form is analyzed. In the dry friction process of silicon nitride ceramics, the coefficient of friction is directly proportional to the contact surface roughness, inversely proportional to the contact load, and directly proportional to the friction speed. There is a critical value for the roughness of friction sub-contact surface. Silicon nitride ceramics can self-lubricate by friction when the contact surface roughness is less than this critical value. In the dry friction process, the oxidation of SiO2 has little effect on the friction. The wear surface of silicon nitride ceramics consists of shear failure units and tensile failure units, and their formation is related to the surface roughness. The results play an important role in revealing the frictional properties of engineering ceramics such as silicon nitride, as well as helpful to improve the wear resistance and service life of ceramic materials.

No abstract

Glioblastoma has high recurrence, while the sensitivity of recurrent glioblastoma to chemotherapy is lower than that of primary glioblastoma. Moreover, there is no standardized treatment for recurrent glioblastoma. Unfortunately, the biological mechanism of recurrent glioblastoma is still unclear, and there are few related studies. We compared the phenotypes of clinical glioblastoma specimens, in-vitro cultured glioma stem-like cells (GSCs) and patient-derived xenograft tumor (PDX) models to explore the molecular genetic characteristics of primary and recurrent glioblastoma from the same patient. In vitro , SU5-2, GSCs derived from recurrent glioblastoma specimens, had stronger proliferative activity and self-renewal ability. Meanwhile, SU5-2 was more resistant to temozolomide and invasive than SU5-1, which derived from primary glioblastoma specimens. Further analysis of the expression of costimulatory molecules showed that the expression of B7-H1, B7-H2 and B7-H3 of SU5-2 were upregulated. In vivo, Kaplan–Meier survival curve analysis showed that the median survival of the recurrent PDX group was worse. The results of gene detection in vitro , PDX model and clinical samples were consistent. Our results showed that the GSCs based on glioblastoma specimens and the PDX models could replicate the main molecular genetic characteristics of original tumors, which provided a reliable experimental platform for both tumor translation kinds of research and screening of molecular therapeutic targets.

Introduction: Clinical metagenomic next-generation sequencing (mNGS) has proven to be a powerful diagnostic tool in pathogen detection. However, its clinical utility has not been thoroughly evaluated. Methods: In this single-center prospective study at the First Affiliated Hospital of Soochow University, a total of 228 samples from 215 patients suspected of having acute or chronic infections between June 2018 and December 2018 were studied. Samples that met the mNGS quality control (QC) criteria (N = 201) were simultaneously analyzed using conventional tests (CTs), including multiple clinical microbiological tests and real-time PCR (if applicable). Results: Pathogen detection results of mNGS in the 201 QC-passed samples were compared to CTs and exhibited a sensitivity of 98.8%, specificity of 38.5%, and accuracy of 87.1%. Specifically, 109 out of 160 (68.1%) CT?/ mNGS? samples exhibited concordant results at the species/genus level, 25 samples (15.6%) showed overlapping results, while the remaining 26 samples (16.3%) had discordant results between the CT and mNGS assays. In addition, mNGS could identify pathogens at the species level, whereas only the genera of some pathogens could be identified by CT. In this cohort, mNGS results were used to guide treatment plans in 24 out of 41 cases that had available follow-up information, and the symptoms were improved in over 70% (17/24) of them.Jie Xu and Peng Zhou have contributed equally to this work.

The removal mechanism of silicon nitride ceramic under high-speed grinding is studied in this article, and grinding parameters are optimized. The efficiency of grinding is improved. The diamond grains of grinding wheel are simplified into the truncated octahedron and cone to simulate the grinding process. When the ceramic workpiece is grinded by singlediamond grain and continuously grinded by multi-diamond grains, the effects of grinding depth and grinding speed on grinding force and surface morphology are analyzed. Through the grinding experiment of the inner surface of silicon nitride ceramic, the grinding force data and surface morphology images were obtained. It proves that at the process of studying the effect of grinding speed on the grinding force and machining surface quality, the results of simulation using multi-diamond grains are closer to the experimental results. The results prove that by increasing wheel speed or lowering grinding depth, the grinding force would be reduced and the quality of grinding surface would be improved. Between grinding depth and grinding speed, the latter factor has a greater impact on grinding force and the quality of grinding surface.

Osteosarcoma often occurs in children and adolescents and affects their health. The survival rate of osteosarcoma patients is unsatisfactory due to the lack of early detection and metastasis development and drug resistance. Hence, dissection of molecular insight into osteosarcoma initiation and progression is pivotal to provide the new therapeutic strategy. In recent years, long noncoding RNAs (lncRNAs) have burst into stage in osteosarcoma development and malignant behaviors. LncRNA SCAMP1 has been discovered to play an essential role in carcinogenesis and progression. However, the mechanisms of lncRNA SCAMP1-involved tumorigenesis have not been reported in human osteosarcoma. In this study, we utilized multiple cellular biological approaches to determine the function of lncRNA SCAMP1 in osteosarcoma cells. Moreover, we performed several molecular biological approaches to define the mechanism by which lncRNA SCAMP1 regulated cell viability and invasion in osteosarcoma. We dissected that lncRNA SCAMP1 promoted progression of osteosarcoma via modulation of miR-26a-5p/ZEB2 axis. In conclusion, targeting lncRNA SCAMP1 and its downstream targets, miR-26a-5p and ZEB2, might be a useful approach for osteosarcoma therapy.

ObjectiveMetagenomic next-generation sequencing (mNGS) technology has the potential to detect a wide range of pathogenic microorganisms. However, reports on the diagnostic value and clinical significance of different platforms of mNGS for patients with lower respiratory tract infections (LRTIs) remain scarce.MethodsA total of 306 patients with suspected LRTIs were enrolled from January 2019 to December 2021. The diagnostic performance of conventional methods and mNGS on bronchoalveolar lavage fluid (BALF) were compared. BALF mNGS was performed using a commercial and an in-house laboratory. The diagnostic value and the clinical implications of mNGS for LRTIs were analyzed for the different platforms.ResultsThe positive rate of mNGS in the in-house group was higher than that in the commercial group (85.26% vs. 70.67%, p < 0.001). mNGS significantly increased the pathogen detection rate compared with conventional methods [from 70.67% vs. 22.67% (p < 0.001) to 85.26% vs. 30.77% (p < 0.001)]. The pathogens detected using mNGS included bacteria, fungi, viruses, and atypical pathogens. The in-house platform performed well on a wider spectrum of microbial distribution. Furthermore, it showed an advantage in detecting mixed pathogens in immunocompromised patients. Among the mNGS positive cases, 34 (32.0%) cases had their antibiotics adjusted in the commercial group, while 51 (38.3%) cases had a change of treatment in the in-house group. Moreover, the turnaround time of mNGS and the time from mNGS to discharge in the in-house group were significantly shorter than those in the commercial group.ConclusionIn-house mNGS had a higher detection rate and can show a wider spectrum of pathogens, with potential benefits for the clinic by shortening the turnaround time and hospitalization, and it may be more suitable for clinical microbiology laboratories.

Different grinding parameters of silicon nitride ceramic are researched at the process of internal grinding. The influences on the roughness of surface by the linear speed of grinding wheel (vs), radial feeding speed (f) and axial vibration speed (fa) are studied in the uniformity testing. The experimental equation of silicon nitride ceramic internal grinding is optimized. The machined surface morphologies of the ceramic specimen are measured by roughness instrument of Taylor-Hobson Surtroni-25 and scanning electron microscope of S-4800. The results show that with the increasing of the linear speed of grinding wheel the roughness of surface get reduced, and with the increasing of radial feeding speed the roughness of surface get raised. It is also inversely proportional to the axial vibration speed. The linear speed of grinding wheel has the biggest impact on the roughness of surface of the silicon nitride ceramic internal grinding among the three grinding parameters. With the increasing of linear speed of grinding wheel, the value of roughness of surface declines from 0.4095μm to 0.1726μm. The influences on the roughness of surface of the silicon nitride ceramic internal grinding by different factors are found out in the research, and its removal mechanisms are cleared up under different linear speeds of grinding wheel.