A powerful rhetoric regarding the importance of adolescents’ civic engagement and political participation is common in contemporary societies, whilst citizens, both adolescent and adults, seem to express a growing scepticism and alienation regarding politics. Even if this disengagement is debatable, as there are simultaneous signs of an increasing involvement in a variety of emerging and broadly‐defined civic and political activities, we argue that the benefits of these experiences should be scrutinized using psychological evidence‐based criteria. We rest on classical contributions from developmental psychology, educational theory and political science to define criteria that could inform the quality of participation experiences, and then present two studies that explore its adequacy. Study 1 is a cross‐sectional study that observes that higher quality civic and political experiences are connected with more complex modes of thinking about politics. In Study 2, a two‐wave longitudinal design, the quality of participation experiences is a significant predictor of change patterns of political attitudes; moreover, results support the argument that participation is not good in itself and that some experiences, with lesser developmental quality, might have a detrimental effect on adolescents’ political development.

Group B Streptococcus (GBS) is the leading cause of neonatal pneumonia, septicemia, and meningitis. We have previously shown that in adult mice GBS glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an extracellular virulence factor that induces production of the immunosuppressive cytokine interleukin-10 (IL-10) by the host early upon bacterial infection. Here, we investigate whether immunity to neonatal GBS infection could be achieved through maternal vaccination against bacterial GAPDH. Female BALB/c mice were immunized with rGAPDH and the progeny was infected with a lethal inoculum of GBS strains. Neonatal mice born from mothers immunized with rGAPDH were protected against infection with GBS strains, including the ST-17 highly virulent clone. A similar protective effect was observed in newborns passively immunized with anti-rGAPDH IgG antibodies, or F(ab')2 fragments, indicating that protection achieved with rGAPDH vaccination is independent of opsonophagocytic killing of bacteria. Protection against lethal GBS infection through rGAPDH maternal vaccination was due to neutralization of IL-10 production soon after infection. Consequently, IL-10 deficient (IL-10−/−) mice pups were as resistant to GBS infection as pups born from vaccinated mothers. We observed that protection was correlated with increased neutrophil trafficking to infected organs. Thus, anti-rGAPDH or anti-IL-10R treatment of mice pups before GBS infection resulted in increased neutrophil numbers and lower bacterial load in infected organs, as compared to newborn mice treated with the respective control antibodies. We showed that mothers immunized with rGAPDH produce neutralizing antibodies that are sufficient to decrease IL-10 production and induce neutrophil recruitment into infected tissues in newborn mice. These results uncover a novel mechanism for GBS virulence in a neonatal host that could be neutralized by vaccination or immunotherapy. As GBS GAPDH is a structurally conserved enzyme that is metabolically essential for bacterial growth in media containing glucose as the sole carbon source (i.e., the blood), this protein constitutes a powerful candidate for the development of a human vaccine against this pathogen.

Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis.

The literature has recently raised the need to clarify the nature of psychological empowerment. There are theoretical and empirical evidences that this conceptualization may not be appropriate, and therefore other alternatives to the reflective measurement model should be considered. Consequently, serious problems may arise from the measurement model misspecification, undoubtedly compromising the development of empowerment theory and measurement. This empirical study constitutes an attempt to contribute to this debate, not only testing both reflective and formative models of psychological empowerment, to find the most appropriate modeling approach as a higher‐order multidimensional construct, but also validating a measure that assesses cognitive, emotional, behavioral, and relational components in a Portuguese youth community‐organizing context (N = 861). Confirmatory tetrad analyses results suggest a formative operationalization of psychological empowerment construct. These findings are supported by additional theoretical and empirical considerations. Additionally, the 46‐item index for measure psychological empowerment reveals satisfactory psychometric properties.

Interactions of several microbial pathogens with the plasminogen system increase their invasive potential. In this study, we show that Streptococcus agalactiae binds human plasminogen which can be subsequently activated to plasmin, thus generating a proteolytic bacterium. S. agalactiae binds plasminogen via the direct pathway, using plasminogen receptors, and via the indirect pathway through fibrinogen receptors. The glyceraldehyde-3-phosphate dehydrogenase is one of the S. agalactiae proteins that bind plasminogen. Presence of exogenous activators such as uPA and tPA are required to activate bound plasminogen. Results from competitive inhibition assays indicate that binding is partially mediated through the lysine binding sites of plasminogen. Following plasminogen binding and activation, S. agalactiae is able to degrade in vitro fibronectin, one of the host extracellular matrix proteins. Moreover, incubation of S. agalactiae with either plasminogen alone, or plasminogen plus fibrinogen, in the presence of tPA enhanced its virulence in C57BL/6 mice, suggesting that acquisition of plasmin-like activity by the bacteria increase their invasiveness.

Objective: Many studies point to the negative impact of discrimination on the psychological and social functioning of ethnic minority persons. The realization of these studies across multiple European countries enables a systematization of this knowledge, which is our goal in the present work. Method: This study presents a meta-analysis of the correlates of perceived ethnic discrimination among minorities living in European countries. Results: The analyses of 121 effect sizes reveal that discrimination is positively associated with symptoms of psychiatric disturbances, depression, psychosis, perceived stress, and externalizing behavior. Discrimination is also negatively associated with the self-esteem, positive evaluation of life, self-efficacy/mastery, well-being, and psychological adaptation of migrants. The results show that the strength of these significant relationships is, in some cases, moderated by persons' gender, age and ethnicity and countries' multicultural approach and rating in the Migrant Integration Policies Index (MIPEX), namely in regards to labor market mobility, permanent residence, and anti-discrimination policies. Conclusions: The detrimental effect of discrimination on many psychosocial dimensions emphasizes the need for governmental agencies to develop systemic and concrete interventions to decrease ethnic prejudice in Europe. Moreover, the results show that fostering multiculturalism, implementing broad anti-discrimination policies and enabling labor market mobility has a protective function in the face of discrimination as fundamental ways to promote the psychological adjustment of ethnic minority persons.

Group B Streptococcus (GBS) is the leading cause of meningitis in neonates. We have previously shown that plasminogen, once recruited to the GBS cell surface and converted into plasmin by host-derived activators, leads to an enhancement of bacterial virulence. Here, we investigated whether plasmin(ogen) bound at the GBS surface contributes to blood-brain barrier penetration and invasion of the central nervous system. For that purpose, GBS strain NEM316 preincubated with or without plasminogen plus tissue type plasminogen activator was analyzed for the capacity to adhere to, invade and transmigrate the human brain microvascular endothelial cell (hBMEC) monolayer, and to penetrate the central nervous system using a neonatal mouse model. At earlier times of infection, plasmin(ogen)-treated GBS exhibited a significant increase in adherence to and invasion of hBMECs. Later, injury of hBMECs were observed with plasmin(ogen)-treated GBS that displayed a plasmin-like activity. The same results were obtained when hBMECs were incubated with whole human plasma and infected with untreated GBS. To confirm that the observed effects were due to the recruitment and activation of plasminogen on GBS surface, the bacteria were first incubated with epsilon-aminocaproic acid (εACA), an inhibitor of plasminogen binding, and thereafter with plasmin(ogen). A significant decrease in the hBMECs injury that was correlated with a decrease of the GBS surface proteolytic activity was observed. Furthermore, plasmin(ogen)-treated GBS infected more efficiently the brain of neonatal mice than the untreated bacteria, indicating that plasmin(ogen) bound to GBS surface may facilitate the traversal of the blood-brain barrier. A higher survival rate was observed in offspring born from εACA-treated mothers, compared to untreated mice, and no brain infection was detected in these neonates. Our findings suggest that capture of the host plasmin(ogen) by the GBS surface promotes the crossing of the blood-brain barrier and contributes to the establishment of meningitis.

Cross-border mobility is one of the most important factors that are assumed to strengthen young people's commitment as European citizens. However, the existing empirical evidence does not provide consistent support. In this paper, we tested the hypothesis that cross-border mobility is associated with a stronger European identification, more positive attitudes toward the EU, and with specific visions of the EU, and that these factors, in turn, have a positive effect on engagement at EU level. Data were collected as part of the multinational research project CATCH-EyoU. Analyses were performed controlling for gender, income, country, and migrant status. Results mostly confirmed the hypotheses among both adolescents and young adults, and for both short-term and long-term mobility. EU level participation and EU voting intentions appeared to be influenced by partly different factors. The implications of the findings, both at theoretical level and for the development of international mobility programs, are discussed.