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Low temperatures represent a crucial environmental factor determining winter survival (WS) of barley and wheat winter-type varieties. In laboratory experiments, low temperatures induce an active plant acclimation response, which is associated with an enhanced accumulation of several stress-inducible proteins including dehydrins. Here, dehydrin accumulations in sampled wheat (WCS120 protein family, or WCS120 and WDHN13 transcripts) and barley (DHN5 protein) varieties grown in two locations for two winters were compared with the variety WS evaluated by a provocation wooden-box test. A high correlation between dehydrin transcripts or protein relative accumulation and variety WS score was found only in samples taken prior vernalization fulfillment, when high tolerant varieties accumulated dehydrins earlier and to higher level than less tolerant varieties, and the plants have not yet been vernalized. After vernalization fulfillment, the correlation was weak, and the apical development indicated that plants reached double ridge (DR) in barley or stayed before DR in wheat. Dehydrin proteins and transcripts can be thus used as reliable markers of wheat or barley variety winter hardiness in the field conditions; however, only at the beginning of winter, when the plants have not yet finished vernalization. In wheat, a higher correlation was obtained for the total amount of dehydrins than for the individual dehydrin proteins.HIGHLIGHTSMore tolerant winter-type wheat and barley plants reveal higher threshold induction temperatures for dehydrin accumulation in comparison to less tolerant varieties. Thus, more tolerant winter cereals have higher dehydrin levels than the less tolerant ones upon the same ambient temperature in November samplings.A significant correlation between dehydrin transcript/protein accumulation and winter survival was found in both winter wheat and winter barley plants in the field conditions, but only prior to vernalization fulfillment.

Spike characteristics include spike length, total spikelet number per spike, number of fertile flowers, spike density, spike fertility, grain number, thousand kernel weight, the number of spikes per square meter, harvest index and the grain yield during the flowering and ripening stage. The six winter and one facultative variety differed in earliness, derived in part from the allele of the Ppd-D1 gene and phenological observation. The two sites significantly differed in the soil moisture, which varied during continual microclimate monitoring. The spike architecture of winter wheat was affected by drought. The plant samples from the site FIELD 2 (more drought stressed) showed a higher reduction in spike characteristics such as a lower spike length, total spikelet number, number of fertile flowers and spike fertility, leading to a lower yield than the site FIELD 1. Both early and late varieties possess compensatory abilities to create the grain yield during drought stress; however, the timing and duration of exposure to drought determine the application and success of the compensatory ability. In our experiment, the late varieties (photoperiod sensitive) performed better in yield than the early varieties during both growing seasons. That is at odds with the generally recommended “drought escape strategy” (early varieties) and suggests a possible direction for variety selection and breeding in arid areas in Central Europe.

Heat stress around anthesis is considered to have an increasing impact on wheat yield under the ongoing climate change. However, the effect of high temperatures and their duration on formation of individual yield parameters is still little understood. Within this study, the effect of high temperatures applied during anthesis for 3 and 7 days on yield formation parameters was analysed. The study was conducted in growth chambers under four temperature regimes (daily temperature maxima 26, 32, 35 and 38°C). In the periods preceding and following heat stress regimes the plants were cultivated under ambient weather conditions. The number of grains per spike was reduced under temperatures ≥ 35°C in cv. Bohemia and ≥ 38°C in cv. Tobak. This resulted in a similar response of spike productivity. Thousand grain weight showed no response to temperature regime in cv. Tobak, whereas in cv. Bohemia, a peak response to temperature with maximum at 35°C was observed. The duration of heat stress had only little effect on most yield formation parameters.