No abstract

Homeostatic mechanisms preventing the toxicity of free Zn ions in cells involve, among others, cytosolic Zn-binding ligands, particularly the cysteine-rich metallothioneins (MTs). Here we examined the Zn-binding peptides of Russula atropurpurea, an ectomycorrhizal fungus known for its ability to accumulate high amounts of Zn in its sporocarps. The Zn complexes and their peptide ligands were characterized using chromatography, electrophoresis after fluorescent labeling of cysteine residues, and tandem mass spectrometry. Functional complementation assays in Saccharomyces cerevisiae were used to obtain and characterize cDNA sequences. Zn-speciation analysis showed that nearly 80% of the Zn extracted from the sporocarps was associated with cysteine-containing peptides in a 5 kDa complex. Screening of an R. atropurpurea cDNA library for sequences encoding peptides capable of sequestering divalent heavy metals was conducted in the Cd-hypersensitive ycf1Δ yeast. This allowed identification of two cDNAs, RaZBP1 and RaZBP2, which protected the metal-sensitive yeast mutants against Cd and Zn, but not Co, Mn or Cu, toxicity. The corresponding RaZBP1 and RaZBP2 peptides consisting of 53 amino acid (AA) residues and sharing 77% identity showed only a limited sequence similarity to known MTs, particularly due to the absence of multiple Cys-AA-Cys motifs. Both RaZBPs were detected in a native Zn-complex of R. atropurpurea and the recombinant RaZBP1 was found associated with Zn and Cd in yeasts. Altogether, the results point to an important role of RaZBPs in the handling of a substantial portion of the Zn pool in R. atropurpurea.

The paper deals with the current trends in development of communication infrastructure which is, above all, represented by high-speed Internet connection (broadband). It is focused on conditions of the Czech Republic; or more precisely, on its rural areas, including possibilities of further development in the following period in context of trends in the world, in the OECD and the EU countries.

The paper presents selected information on the newest results of a wide investigation of the state of information and communication technologies development in agricultural production enterprises in the Czech republic. The investigation was realized in the first half of 2009 with the main aim to analyze development of information infrastructure and actual trends in iCt use in rural regions where most entrepreneurial subjects operate. Besides a presentation of own research results, some obtained pieces of knowledge are then commented and eventually discussed in connection with official outputs of the Czech Statistical office (CSo), if they have a certain relevance to the given problems.

Potential of woody species vegetation components is defined as the total ability of existing woody species vegetation components (hereinafter referred to as WSVC) of a particular object (or its parts) to ensure the composition stability. The main criteria for evaluation of this potential at all levels are: (a) current (relevant) characteristics of WSVC; (b) expected development of ability of WSVC to preserve, renew and develop its desirable characteristics; (c) expected length of biologically conditioned existence of WSVC (in an acceptable condition); and (d) expected length of desirable existence of WSVC.Evaluation is based on mutual confrontation and interpretation of especially these woody species characteristics: taxon, age or development stage and landscaping value. These characteristics are surveyed during the evaluation of woody species as standard in the Czech Republic, or at least at a fairly regular basis. It proceeds from the lowest level (individual) to the highest level (the whole object). Especially in case of larger objects and their parts is used as a basis the spatial distribution of the aforementioned characteristics in the evaluated unit (terrain layout) as well as statistical evaluation of these characteristics.The focuses of this paper are methodological issues of determining and expressing the potential of woody species components.

No abstract

The paper deals with a general solution of semantic description related to various electronic data formats in the domains of agriculture, aquaculture, forestry, food industry, environment, horticulture and rural areas. The solution presented was developed on the basis of metadata formats analysis and then complemented with its own software superstructure. It is based on the VOA 3 R metadata application profile (AP) that was developed within the framework of the Virtual Open Access Agriculture and Aquaculture Repository project for the sake of describing research papers and scientific publications. However, thanks to its complexity and comprehensiveness, it is also suitable for different kinds of data and while combining it with the AGROVOC thesaurus, which is elaborated by the Food and Agriculture Organization of the United Nations, it can become a robust and universal tool for data characteristics and semantic description. The potential of the proposed solution is illustrated by means of two examples. While the first example brings a metadata description of a photograph depicting anthocyanin pigmentation of barley straw caused by phosphorus deficiency, the second example is related to a research paper description. The developed system of metadata description is broadly applicable in agriculture such as in precision agriculture, in plant production or in ground cover monitoring and evaluation based on sensor or visual data.

Rural areas in Europe are at risk due to depopulation, failing generation renewal, and a multitude of influences ranging from market-based, regulatory, to societal and climate changes. As a result, current rural policy is no longer keeping pace with these changes. We propose an advanced rural policy development framework in order to deliver more accurate foresight for rural regions, contributing to new and enhanced policy interventions. The proposed framework combines new quantitative and qualitative epistemological approaches, previously unused unstructured data with traditional research information, grassroot perspective with expert knowledge, current situation analysis with forward looking activities. We argue that by using the proposed methods, policy teams will be able to enhance the effectiveness of their policy making processes, while rural stakeholders will be given the opportunity to become valuable policy influencers and solution co-creators. The ability to quickly experiment and understand the impact of a variety of policy solutions will result in saved time and costs. The framework is part of an ongoing experimental verification and testing in twelve pilot regions across Europe and Israel.