A method for phytic acid determination in the feeds and faeces of pigs and poultry has been developed on the basis of capillary isotachophoresis. Phytic acid was extracted by 0.95 M HCl and separated from interfering compounds by iron precipitation. Complete formation of ferric phytate required 7 mol FeCl 3 mol À1 phytic acid. Residual Fe 3 was estimated colorimetrically by the tiron reagent, and ferric phytate was dissolved in 1.5 M NaOH at 9 mol NaOH mol À1 Fe precipitated. Analyses were carried out using an electrolyte system with Cl À as the leading anion, bis-tris-propane, and 2-morpholinoethanesulphonic acid as the terminating anion. The recovery of phytic acid (added to hen faeces) using this procedure was 962 AE 24 g kg À1. The limit of determination of phytic acid was 0.3 mmol ml À1 extract. The amount of phytic acid in feeds ranged from 8.3 to 10.8 g kg À1 on a dry matter basis. Phytic acid P represented 112 g kg À1 total P in faeces of young pigs (40±60 kg) fed a feed with supplemental phytase (490 U kg À1 ), 153 g kg À1 total P in faeces of ®nishing pigs and 185 g kg À1 total P in faeces of non-lactating sows. Excreta of laying hens contained 23.7 g phytic acid kg À1 dry matter (362 g kg À1 total P). The isotachophoretic method is suf®ciently simple and reproducible to be used for routine analyses of feeds and faeces. # 2000 Society of Chemical Industry Keywords: phytic acid; isotachophoresis; feed; faeces; pig; hen INTRODUCTIONPhytic acid (myo-inositol hexaphosphoric acid) is present in substantial quantities in a variety of plant feedstuffs. It is the predominant form of phosphorus in cereals, oil-seeds and seeds of leguminous plants.
A method for phytic acid determination in the feeds and faeces of pigs and poultry has been developed on the basis of capillary isotachophoresis. Phytic acid was extracted by 0.95 M HCl and separated from interfering compounds by iron precipitation. Complete formation of ferric phytate required 7 mol FeCl3 mol−1 phytic acid. Residual Fe3+ was estimated colorimetrically by the tiron reagent, and ferric phytate was dissolved in 1.5 M NaOH at 9 mol NaOH mol−1 Fe precipitated. Analyses were carried out using an electrolyte system with Cl− as the leading anion, bis‐tris‐propane, and 2‐morpholinoethanesulphonic acid as the terminating anion. The recovery of phytic acid (added to hen faeces) using this procedure was 962 ± 24 g kg−1. The limit of determination of phytic acid was 0.3 µmol ml−1 extract. The amount of phytic acid in feeds ranged from 8.3 to 10.8 g kg−1 on a dry matter basis. Phytic acid P represented 112 g kg−1 total P in faeces of young pigs (40–60 kg) fed a feed with supplemental phytase (490 U kg−1), 153 g kg−1 total P in faeces of finishing pigs and 185 g kg−1 total P in faeces of non‐lactating sows. Excreta of laying hens contained 23.7 g phytic acid kg−1 dry matter (362 g kg−1 total P). The isotachophoretic method is sufficiently simple and reproducible to be used for routine analyses of feeds and faeces. © 2000 Society of Chemical Industry
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