This case report describes a highly pruritic, papular and crusted dermatitis affecting the dorsum of a dog and microscopically diagnosed as dermatitis induced by Straelensia cynotis. Histologically, each papule was characterized by a dilated hair follicle with marked pseudoepitheliomatous hyperplasia, perifollicular mucinosis and an abundant mononuclear infiltration by plasma and mast cells. Each dilated follicle contained a larval mite. A therapy including systemic ivermectins and oral antibiotic therapy was initiated, but the clinical response was poor. To the best of the authors' knowledge, this is the first report of straelensiosis in Portugal, and the second in Europe.
Social barriers have been shown to reduce gene flow and contribute to genetic structure among populations in species with high cognitive capacity and complex societies, such as cetaceans, apes and humans. In birds, high dispersal capacity is thought to prevent population divergence unless major geographical or habitat barriers induce isolation patterns by dispersal, colonization or adaptation limitation. We report that Iberian populations of the red-billed chough, a social, gregarious corvid with high dispersal capacity, show a striking degree of genetic structure composed of at least 15 distinct genetic units. Monitoring of marked individuals over 30 years revealed that long-distance movements over hundreds of kilometres are common, yet recruitment into breeding populations is infrequent and highly philopatric. Genetic differentiation is weakly related to geographical distance, and habitat types used are overall qualitatively similar among regions and regularly shared by individuals of different populations, so that genetic structure is unlikely to be due solely to isolation by distance or isolation by adaptation. Moreover, most population nuclei showed relatively high levels of genetic diversity, suggesting a limited role for genetic drift in significantly differentiating populations. We propose that social mechanisms may underlie this unprecedented level of genetic structure in birds through a pattern of isolation by social barriers not yet described, which may have driven this remarkable population divergence in the absence of geographical and environmental barriers.
Capsule The Skylark Alauda arvensis had the highest overall mortality in ten Northern Portuguese wind farms surveyed between 2006 and 2011. Analysis from the integration of conventional and molecular techniques suggest a sex and age biased mortality affecting mainly adult males (90.9%), which may be related to their characteristic breeding male song-flights making them highly vulnerable to collision with wind turbines. The results highlight the added value of more complete population impact assessments that go beyond simple carcass identification at wind farms.
High-resolution melting (HRM) analysis is a very attractive and flexible advanced post-PCR method with high sensitivity/specificity for simple, fast and cost-effective genotyping based on the detection of specific melting profiles of PCR products. Next generation real-time PCR systems, along with improved saturating DNA-binding dyes, enable the direct acquisition of HRM data after quantitative PCR. Melting behaviour is particularly influenced by the length, nucleotide sequence and GC content of the amplicons. This method is expanding rapidly in several research areas such as human genetics, reproductive biology, microbiology and ecology/conservation of wild populations. Here we have developed a successful HRM protocol for avian sex identification based on the amplification of sex-specific CHD1 fragments. The melting curve patterns allowed efficient sexual differentiation of 111 samples analysed (plucked feathers, muscle tissues, blood and oral cavity epithelial cells) of 14 bird species. In addition, we sequenced the amplified regions of the CHD1 gene and demonstrated the usefulness of this strategy for the genotype discrimination of various amplicons (CHD1Z and CHD1W), which have small size differences, ranging from 2 bp to 44 bp. The established methodology clearly revealed the advantages (e.g. closed-tube system, high sensitivity and rapidity) of a simple HRM assay for accurate sex differentiation of the species under study. The requirements, strengths and limitations of the method are addressed to provide a simple guide for its application in the field of molecular sexing of birds. The high sensitivity and resolution relative to previous real-time PCR methods makes HRM analysis an excellent approach for improving advanced molecular methods for bird sexing.
Summary1. The Eurasian skylark Alauda arvensis is very susceptible to the negative effects of wind farms. In northern Portugal, this evidence is particularly severe due to the skylark's preference for mountain breeding habitats where most wind farms are located. Facing the frequent failure of environmental impact assessments (EIA) to evaluate the cumulative impacts of wind farms on wildlife, this study aimed to develop and test a methodology to quantify local and regional consequences on birds, using skylarks as a test species, taking into account future predictable environmental changes. 2. We propose a spatially explicit dynamic approach that combines the results from multiple modelling techniques under a common framework to assess the local and cumulative regional impacts of wind farms on skylark populations. This includes the following: (i) modelling the local impact of wind farms (in terms of collision mortality) on the skylark population dynamics by developing an index for quantitative assessments, (ii) determining the actual and future skylark breeding distribution across northern Portugal and (iii) integrating the above contributions in an emergent spatially explicit regional representation to capture the ecological cumulative consequences as a whole. 3. The simulations show an increasing average local impact for the skylark breeding populations directly affected by wind farms, expressed in mean number of collision fatalities per UTM study unit (1 km 2 ), representing 1Á3% of the local breeders in 2006 and 4% in 2026.4. The distribution area of skylark breeding populations was predicted to decrease around 4Á5% throughout a period of 15 years, as a result of the scenario of climate and land cover changes in the study area. When combined with a concomitant increase in skylark global mortality (c. 184%) induced by all wind farms in the study region, the above trend contributes to an intensification of the regional cumulative impact from 1Á2% to 3Á7% of the total estimated breeding individuals. 5. Synthesis and applications. The proposed modelling framework represents a step forward in evaluating the multi-scale cumulative consequences of wind farms on vulnerable birds, using skylarks as a test species. This could be used in the future to guide monitoring efforts and to improve the applicability of the data bases generated by long-term ecological research and monitoring studies.
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