Water availability and temperature are among the main abiotic factors that influence seed germination and vigor, since they act directly on biochemical and physiological processes, which result in the production of the primary root. The objective of this study was to verify the effects of stress on germination and vigor of H. impetiginosus (‘ipê-roxo’) seeds under different temperatures and osmotic agents. The experimental design was completely randomized, in a 6 x 2 factorial scheme (osmotic potentials x temperatures) with four replicates of 25 seeds for each osmotic agent. In order to simulate water stress, polyethylene glycol (PEG 6000) and mannitol solutions were used and distilled water was used as control. Osmotic potentials of 0, -0.2, -0.4, -0.6, -0.8 and -1.0 MPa were evaluated at temperatures of 25 and 30 °C, under 8 h photoperiod. The variables analyzed were: germination, germination speed index, shoot length, root length, and total dry matter of seedlings. Simulated water stress affected seed germination and seed vigor of H. impetiginosus at both temperatures and osmotic agents from -0.6 MPa.
Seeds are one of the main inputs used in agriculture, and their quality is directly related to the success of the field crop. Thus, seed companies increasingly need to obtain fast and reliable seed quality results, making it easier to make decisions regarding the use of seed batches. In this sense, the objective was to evaluate the CO2 measurement efficiency in the classification of S. bicolor seed vigor. For this, six batches of cv. Ponta Negra seeds were used, from different national producers. The physiological quality of the seeds was evaluated by means of the following tests: germination, first germination count, germination speed index, accelerated aging, total dry matter of seedlings, electrical conductivity and respiratory activity. The experimental design was completely randomized, with four replications. The Pearson correlation coefficient between the respiratory activity and the germination and vigor tests was determined. The respiratory activity test on S. bicolor seeds was shown to be promising for the identification of differences in vigor between seed batches.
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