Producing animal proteins requires large areas of agricultural land and is a major source of greenhouse gases. Cellular agriculture, especially cultured meat, could be a potential alternative for the environment and human health. It enables meat and other agricultural products to be grown from cells in a bioreactor without being taken from farm animals. This paper aims at an interdisciplinary review of literature focusing on potential benefits and risks associated with cultured meat. To achieve this goal, several international databases and governmental projects were thoroughly analyzed using keywords and phrases with specialty terms. This is a growing scientific domain, which has generated a series of debates regarding its potential effects. On the one hand the potential of beneficial effects is the reduction of agricultural land usage, pollution and the improvement of human health. Other authors question if cultured meat could be a sustainable alternative for reducing gas emissions. Interestingly, the energy used for cultured meat could be higher, due to the replacement of some biological functions, by technological processes. For potential effects to turn into results, a realistic understanding of the technology involved and more experimental studies are required.

Blood biochemistry parameters are valuable tools for monitoring fish health. Their baseline values are still undefined for a multitude of farmed fish species. In this study, changes in the blood profile of rainbow trout females (Oncorhynchus mykiss) from three farms were investigated using different biomarkers during the summer season. In the given context, the main water physicochemical parameters were investigated and twelve biochemical parameters were measured from blood samples of rainbow trout reared in the Fiad, Șoimul de Jos, and Strâmba farms. We selected these farms because the genetic background of the rainbow trout is the same, with all studied specimens coming from the Fiad farm, which has an incubation station. Forty-five samples were collected monthly (May to August) throughout summer to observe the changes in the blood profile of rainbow trout. Principal component analysis showed a clear separation both among the studied farms and months. Furthermore, significant correlations (p < 0.05) between the majority of the biochemical parameters were found, indicating that the environmental parameters can influence several blood parameters at the same time. The present study provides several useful norms for assessing the welfare of rainbow trout, indicating that the relationships among different parameters are important factors in interpreting the blood biochemical profiles.

The parameters studied were RBC, Ht, Hb, erythrocyte index MCV, MCH, MCHC, and oxidative stress through SOD and GPx. For each parameter, blood samples were collected at specimens weighing between 100-140g in the summer season, 250-280 g in the fall, 270-300g in the winter and 320-350g in the spring. At the specimens studied the highest values of GPx and SOD were recorded in the spring season due to the stress caused by massive precipitation. During the study, the water temperature ranged between 5.1° C in winter and 19.2° C in summer and O 2 ranged between 8.30 mg/l in the summer and 10.20 mg / l in winter. For most parameters, the highest values were recorded in the spring season and the lowest in the summer season. The data obtained in each season on Oncorhynchus mykiss species do not indicate pathological conditions but more an adaptive fish response to environmental conditions and can serve as a database for future research on welfare of salmonids.

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The review assembles chronological data on Black Sea trout (Salmo labrax) from Romanian waters and brings up-to-date information related to the distribution of the species. The information used dates from 1909 to 2020 and includes books, articles, digital databases, field observations, and notes from different research fields such as ichthyology, biogeography, genetics, aquaculture, conservation, and ecology. Global distribution, migration, meristic characters, and aquaculture of the species were analyzed based on the recorded data from the specialty literature. New information related to a possible population of Salmo labrax inside the Carpathian Arch was discussed. In Romanian waters the species is found in the Black Sea, Danube, Danube Delta but the current paper proposes a new hypothesis, namely that resident populations can be found in rivers and lakes adjacent to the Carpathian Arch. The highest migration point of the Black Sea trout in the Danube was recorded near Corabia locality, Olt County, (43°46′25″N- 24°30′12″E). In the Danube Delta, it was caught in all the three branches (Sulina, Sf. Gheorghe and Chilia), and lagoonary complexes such as Razim-Sinoe. Sexually matured females were caught especially in Spring Season while unmatured specimens were caught in all seasons. Even if the species is protected under Romanian legislation, fishermen and anglers should report its presence when caught accidentally.

In the present study, changes in fish species composition, distribution, and diversity caused by cascade dam constructions on Someșul Cald River, Romania, were analyzed. Cascade dam constructions may act as a freshwater stressor both on short and long term. The river was divided into two sections according to the positioning of the cascade dams (T1-inferior section, respectively T2-superior section). In the T1 and T2 sections, 8 and 12 sampling stations were established, respectively. A total number of 1789 specimens grouped in 12 species and 8 families were sampled from August to September 2018. The most abundant species in both sections was S. trutta. The most abundant family was Salmonidae, with 66.2%. Alpha diversity (species richness) in the T2 section was higher than in T1 (T2 = 12 species vs T1 = 9 species). The Kruskal-Wallis test indicated no significant differences (p &gt; 0.05) for beta diversity of T1 vs T2 (Sørensen coefficient). The species that contributed most to the dissimilarities between the two river sections were T. thymallus, B. carpathicus, C. gobio, and S. cephalus, their summed contribution to the dissimilarity being larger than 50%. The analyzed environmental variables influenced the distribution of some fish species. Alpha and beta diversity distribution on elevation gradient showed that stations close to reservoirs had more species (both in T1 and T2 sections) than the other stations, clearly showing that the distance from lake/reservoir is influencing species richness. The effect of dams from Someșul Cald River on fish species distribution was analyzed by comparing previous data (before 1968) to recent collected data.

The habitat of the European mudminnow, Umbra krameri Walbaum, 1792, is continuously decreasing due to human intervention. The species has a “vulnerable” status according to the IUCN Red List. Thus, new information about the species is needed for conservation efforts. Minnow traps were used for capturing Umbra krameri and other small fishes in the Jieț River, Dolj County, southwestern Romania. Captured specimens of U. krameri were measured and weighed. Length–weight relations and relative condition factor were determined for the captured U. krameri specimens. A total of 94 fishes were captured using the minnow traps. They represented 7 species: European bitterling, Rhodeus amarus (Bloch, 1782), roach, Rutilus rutilus (Linnaeus, 1758), rudd, Scardinius erythrophthalmus (Linnaeus, 1758), Danubian spined loach, Cobitis elongatoides Băcescu et Mayer, 1969, weatherfish, Misgurnus fossilis (Linnaeus, 1758), tubenose goby, Proterorhinus marmoratus (Pallas, 1814), and U. krameri. The specimens of Umbra krameri represented 44% (41 specimens) of the total catch. The wet body weight (BW) of U. krameri ranged from 0.8 to 5.1 g, having a mean value of 2.102 g. The mean total length (TL) was 5.782 cm, ranging from 4.59 to 7.87 cm. According to the length–weight relation (LWR), the growth type of U. krameri was positive allometric based on the determined equation: BW = 0.0068TL3.277. The mean value determined for the relative condition factor (Kn) was 1.0056486. The growth condition of 46% of the specimens was poor (Kn &lt; 1; n = 19), while the other 54% were in good condition (Kn ≥ 1; n = 22). The presently reported study provides information about the presence of U. krameri in the Jieț River (Dolj County, Romania), a location where the species has not been previously reported. The LWRs show an allometric positive growth. The relative condition factor shows that 46% of the specimens had poor growth, while the rest were in good condition. Minnow traps were an efficient tool for small fish capture.

In this study, we aimed to highlight the influence of anticoagulants on erythrocyte morphometry in different vertebrate species. Anticoagulants are a category of substances that inhibit blood clotting through various mechanisms. Due to this property, they are used to collect blood samples for a wide range of laboratory tests. The literature mentions that the use of anticoagulants produces morphological changes of erythrocytes, thus influencing results. Blood samples were collected from three warm-blooded vertebrate species (horse, rabbit, and chicken) and one lower vertebrate species with nucleated erythrocytes (fish) in vacutainers with Heparin and EDTA (ethylenediaminetetraacetic acid), in a normal concentration and a double concentration. At the time of harvesting, control smears were performed. In order to be able to compare the effects produced by anticoagulants on the morphology of erythrocytes, they were evaluated morphometrically at intervals of 3, 6, and 24 hrs. after harvest. The following features were evaluated using the Toup View software: length, width, surface and perimeter of erythrocytes for species with anucleated erythrocytes. The same characteristics were evaluated in the nucleus for species with nucleated erythrocytes. The data obtained were processed with statistical programs to highlight changes in erythrocyte morphology produced by anticoagulants.