Multi-Approach Assessment for Stress Evaluation in Rainbow Trout Females, Oncorhynchus mykiss (Walbaum, 1792) from Three Different Farms during the Summer Season

Abstract

Blood biochemistry parameters are valuable tools for monitoring fish health. Their baseline values are still undefined for a multitude of farmed fish species. In this study, changes in the blood profile of rainbow trout females (Oncorhynchus mykiss) from three farms were investigated using different biomarkers during the summer season. In the given context, the main water physicochemical parameters were investigated and twelve biochemical parameters were measured from blood samples of rainbow trout reared in the Fiad, Șoimul de Jos, and Strâmba farms. We selected these farms because the genetic background of the rainbow trout is the same, with all studied specimens coming from the Fiad farm, which has an incubation station. Forty-five samples were collected monthly (May to August) throughout summer to observe the changes in the blood profile of rainbow trout. Principal component analysis showed a clear separation both among the studied farms and months. Furthermore, significant correlations (p < 0.05) between the majority of the biochemical parameters were found, indicating that the environmental parameters can influence several blood parameters at the same time. The present study provides several useful norms for assessing the welfare of rainbow trout, indicating that the relationships among different parameters are important factors in interpreting the blood biochemical profiles.

Effects of diets containing β‐glucan on survival, growth performance, haematological, immunity and biochemical parameters of rainbow trout ( Oncorhynchus mykiss ) fingerlings

“…Regarding the amounts of haemoglobin and haematocrit, the lowest amount was observed in the control group. Fish red blood cell and haemoglobin counts change significantly with seasonal changes, sexual cycle and other physiological factors (Uiuiu et al, 2021). Due to the constant environmental conditions and fish, the presence of β‐glucan in the diet affected the haemoglobin concentration.…”

Mitigating the impact of winter temperatures on striped catfish (Pangasianodon hypophthalmus) using functional feed additives

“…Serum samples were collected after centrifuging at 3000 g at 4°C for 15 min and stored at −80°C for later analysis (Refaey & Li, 2018). A commercially available enzyme‐linked immunosorbent assay (ELISA) kit was used for the measurement of serum cortisol levels (#DKO001; Diameter, Milano, Italy, Uiuiu et al, 2021). The determination of serum glucose level was undertaken through the glucose oxidase method using a commercial assay kit (#1000178, Pars Azmun, Tehran, Iran).…”

Changes in Serum Blood Parameters in Farmed Rainbow Trout (Oncorhynchus mykiss) Fed with Diets Supplemented with Waste Derived from Supercritical Fluid Extraction of Sweet Basil (Ocimum basilicum)

“…Hematobiochemical analysis is useful in diagnostics for predicting the health status of farmed fish species [37,39], and blood biochemistry parameters are basic physiological markers in fish [38]. Previous studies have investigated changes in blood chemistry in response to disease [40], contaminants [41,42], parasitic infestation [43], environmental stressors [44], inappropriate stocking density [45,46], and farm practices [47].…”