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SUMMARY: A lethal oedema-producing toxin has been made in vitm in serum cultures of Bacillus anthracis which is identical with the toxin originally recognized in Wiuo. Toxin is produced by the attenuated immunogenic Sterne strain as well as by the virulent N.P. strain. Previous non-recognition of the toxin in cultures was due to its early appearance and rapid disappearance under ordinary growth conditions. A toxin-destroying mechanism was demonstrable in organisms from cultures grown for more than a few hours. Attempts to maintain the toxin concentration or to increase it by addition of various nutrilites to batch culture failed; only by continuous culture have the organisms been kept in their toxigenic growth phase. "races of the toxin appear in cultures in tryptic meat broth; these are increased by the addition of large molecular constituents of serum which appear to play an important role in toxin production.

Summary Normal values for a number of blood components of grivet monkeys are reported. Haematological data and values for glucose, cholesterol and urea are similar to those of rhesus monkeys. Activities of alkaline phosphatase (1526 U/l), glutamine oxaloacetate trans-aminase (30·9 U/l), glutamine pyruvate transaminase (13·7 U/l), lactate dehydrogenase (629 U/l), α-hydroxybutyrate dehydrogenase (175 U/l), creatine phosphokinase (227 U/l), γ-glutamyl transpeptidase (38·7 U/l) and sorbitol dehydrogenase (14·2 U/l), and levels of lysozyme (178 mg/dl), zinc (162 μg/dl), copper (81·3 μg/dl) and iron (296·5 μg/dl) have not previously been reported for this animal. Values for serum amino acids, proteins, electrolytes, triglycerides and creatinine are compared with those of other primates.

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