Affinity-Purified Ulex europaeus Lectin: Preparation and Serological Characteristics

Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping

“…The latter group noted that inhibition tests with the anti-B were four dilutions more sensitive with A1B than with B cells. Harris-Smith and Fletcher (1983) obtained good inhibition results with 0 secretor saliva using an A P U E A I. The inhibitive titers with the A P reagent and the conventional anti-H in their studies were comparable to those obtained in the present work.…”

“…Voak et al (1983) carried out absorption elution studies on A1 and A2 cells with MC anti-A and on B cells with MC anti-B, and obtained good eluate titers. Harris-Smith and Fletcher (1983) compared an AP U E A I reagent with conventional anti-H in absorption elution tests on group 0 bloodstained cloth using the Howard-Martin (1969) technique and on dried ammoniacal extracts of 0 bloodstains (Kind and Cleevely 1969). Eluates obtained with the AP reagent Table 5.…”

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells

“…Thus, purification of the specific antibodies is necessary for shortening the time required and for lowering the blank values in the method. The purification of anti-A or anti-B antibodies is usually made by affinity chromatography [2][3][4][5] requiring purified blood-group substances.…”