Polyhydroxyalkanoates
(PHAs) are biological polyesters that can
be naturally produced by a range of bacteria as water-insoluble inclusions
composed of a PHA core coated with PHA synthesis, structural, and
regulatory proteins. These naturally self-assembling shell–core
particles have been recently conceived as biomaterials that can be
bioengineered as biologically active beads for medical applications.
Protein engineering of PHA-associated proteins enabled the production
of PHA–protein assemblies exhibiting biologically active protein-based
functions relevant for applications as vaccines or diagnostics. Here
we provide an overview of the recent advances in bioengineering of
PHA particles toward the display of biomedically relevant protein
functions such as selected disease-specific antigens as diagnostic
tools or for the design of particulate subunit vaccines against infectious
diseases such as tuberculosis, meningitis, pneumonia, and hepatitis
C.
BackgroundLaboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface of polyester (polyhydroxyalkanoate, PHA) beads in vivo in Escherichia coli by genetically fusing them to a polyester synthase gene (phaC). The insertion of a self-cleaving module, a modified sortase A (SrtA) from Staphylococcusaureus and its five amino acid recognition sequence between the synthase and the target protein led to a simple protein production and purification method.ResultsThe generation of hybrid genes encoding tripartite PhaC-SrtA-Target fusion proteins, enabled immobilization of proteins of interest to the surface of PHA beads in vivo. After simple cell lysis and isolation of the PHA beads, the target proteins could be selectively and efficiently released form the beads by activating the sortase with CaCl2 and triglycine. Up to 6 mg/l of soluble proteins at a purity of ~98 % could be isolated in one step with no optimization. This process was used to produce and isolate three proteins: Green fluorescent protein, maltose binding protein and the Mycobacteriumtuberculosis vaccine candidate Rv1626.ConclusionsWe have developed a new technique for easy production and purification of recombinant proteins. This technique is capable of producing and purifying high yields of proteins suitable for research application in less than 2 days. No costly or specialized protein chromatography equipment, resins, reagents or expertise are required.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-015-0385-3) contains supplementary material, which is available to authorized users.
Despite recent advances in diagnosis, tuberculosis (TB) remains one of the ten leading causes of death worldwide. Here, we engineered Mycobacterium tuberculosis (Mtb) proteins (ESAT6, CFP10, and MTB7.7) to self-assemble into core-shell nanobeads for enhanced TB diagnosis.Respective purified Mtb antigen-coated polyester beads were characterized and their functionality in TB diagnosis was tested in whole blood cytokine release assays. Sensitivity and specificity were studied in 11 pulmonary TB patients (PTB) and 26 healthy individuals composed of 14 Tuberculin Skin Test negative (TSTn) and 12 TST positive (TSTp). The production of 6 cytokines was determined (IFNγ, IP10, IL2, TNFα, CCL3, and CCL11). To differentiate PTB from healthy individuals (TSTp + TSTn), the best individual cytokines were IL2 and CCL11 (>80% sensitivity and specificity) and the best combination was IP10 + IL2 (>90% sensitivity and specificity). We describe an innovative approach using full-length antigens attached to biopolyester nanobeads enabling sensitive and specific detection of human TB.
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