Bacterially assembled biopolyester nanobeads for removing cadmium from water

Antimicrobial peptide-functionalized polyhydroxyalkanoate bio-beads as a bactericidal material for water disinfection

“…Overall, the FTIR spectra profiles of both BDH and control bio-beads are in good agreement with those of PHA granules synthesized by C. necator and other engineered E. coli strains. 54,61…”

“…[47][48][49] The PHA display platform allows for simultaneous PHA particle formation, target protein/peptide production and immobilization in a one-pot manner. The platform has been used in research studies to create biomaterials for industrial biotechnology and environmental remediation applications, [50][51][52][53][54] but its application in creating antimicrobial biomaterials for water disinfection has not been explored.…”

Bioengineered Polymer Nanobeads for Isolation and Electrochemical Detection of Cancer Biomarkers

“…coli) or Bacillus megaterium were engineered to produce surface-functionalized PHB beads enabling low-cost manufacturing processes and the development of molecular tools to precision-engineer high-performance materials for uses such as in bioassays. ,− Bioengineered PHB nanobeads are core–shell structures where the core is made of semicrystalline hydrophobic PHB exhibiting unique thermal and mechanical properties while the surface is composed of covalently anchored proteins that can be engineered to densely present binding domains in a homogeneous orientation facilitating access to target molecules suitable for biosensing applications (Figure ). ,, The self-assembly of PHB-anchored proteins translationally fused to protein functions during PHB synthesis enables the dense and oriented display of various protein functions on the surface for enhanced target capture and recognition. − The increased functional surface area of PHB nanobeads also allows for integration of inorganic binding peptides that mediate binding such as gold, silica, cadmium, and, as described in this study, iron oxide to convey additional physical properties such as superparamagnetism. Here, we bioengineered E.…”

“…3,13,14 The selfassembly of PHB-anchored proteins translationally fused to protein functions during PHB synthesis enables the dense and oriented display of various protein functions on the surface for enhanced target capture and recognition. 14−16 The increased functional surface area of PHB nanobeads also allows for integration of inorganic binding peptides that mediate binding such as gold, silica, 10 cadmium, 17 as superparamagnetism. Here, we bioengineered E. coli to assemble PHB nanobeads that were able to specifically bind any antibody via its Fc domain and that could be processed into superparamagnetic nanobeads.…”

Intranasal Delivery of Antigen-Coated Polymer Particles Protects against Pseudomonas aeruginosa Infection

“…These measurements showed a size distribution previously described for BPs. 40,41,43,44 The formulation of BPs with Alhydrogel increased the size range to 1.71−4.05 μm presumably because of the size of Alhydrogel itself (1.79 μm) and via the ligandexchange mechanism or electrostatic interactions (between particles or their hybrids). 45 The negative surface charge of BP, BP-Ag, BP-PopB, BP-PopBAg, BP-10Ag, and BP-10Ag(2x) was expected because of the theoretical isoelectric points (pI) of PhaC (6.08 pI), Ag (9.84 pI), PopB (6.35 pI), PopBAg (9.12pI), PAB1 and PAB2 (9.15 and 9.24 pI), and PA1 and PA2 (9.26 and 9.00 pI) proteins coating the BPs.…”