Background: Studies carried out with novel 13 strains of Trichoderma, isolated from mangrove sediments (PE, Brazil) using morphophysiological and molecular characterization, followed evaluation of biocontrol using Fusarium strains isolated from Caatinga soil (PE, Brazil). Trichoderma strains were characterized by polyphasic taxonomic approach, and the extracted DNA was amplified with primers ITS 1 and 4, and sequenced. The biocontrol evaluation was conducted at 24 and 48 h of growth intervals by Tukey test, with a significance of 5%. Antibiosis tests were assessed in vitro by dual plate and partition plate techniques against Fusarium strains. Results: Trichoderma molecular identification, sequences of 500 bp were amplified, deposited into GenBank, and used for phylogenetic analyses. The strains were identified as T. asperellum (10), as T. harzianum (2) and one as T. longibrachiatum. Growth rate presented an average of 0.1207 cm h −1 for Trichoderma and lower growth rate of 0.031 cm h −1 for Fusarium spp., respectively. Antibiosis tests presented the best antagonist level of efficiency for T. asperellum UCP 0149 against F. solani UCP 1395 (82.2%) and F. solani UCP 1075 (70.0%), followed by T. asperellum UCP 0319 against F. solani UCP1083 (73.4%) and T. asperellum UCP 0168 against F. solani UCP1098 (71.5%), respectively. Conclusions: The data obtained in this study as tool for identification of novel Trichoderma strains serve as basis for development of several sustainable use for biotechnological processes. Those Trichoderma strains found promising for the management antagonistic potential and interaction could aid the conduct of biotechnological biocontrol of contaminants, and improve environmental conditions for the health of plants.
Palavras-chaveEpidemiologia , estudo de populações, alcoolismo. rEsumo objetivo: Esse inquérito epidemiológico verificou a prevalência de alcoolismo e o uso de álcool nas famílias mais antigas e numerosas da população da ilha de Fernando de Noronha, estado de Pernambuco, Brasil, e tentou identificar o perfil sociodemográfico associado a este problema naquela região. métodos: A amostra representativa da população foi composta por 119 pessoas. O instrumento de investigação incluiu perguntas sobre os dados sociodemográficos da amostra e caracterização do consumo de álcool. Para avaliar a prevalência de alcoolismo, entre estes indivíduos, utilizou-se instrumento de rastreamento para distúrbios relacionados ao álcool -o CAGE -, levando-se em conta o ponto de corte de uma ou mais respostas positivas para definir alcoolistas. resultados: Observou-se prevalência de consumo global de álcool em 62,2% da amostra e de alcoolismo em 40,34%, sendo 50,9% para homens e 30,6% para mulheres. Homens, solteiros, separados e viúvos não praticantes da religião protestante apresentaram risco significativamente mais elevado de alcoolismo nessa região. Conclusão: Os resultados obtidos foram comparados com estudos anteriores em outras populações e novas linhas de pesquisa são sugeridas.
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