Objective
During the current pandemic, COVID-19 has been detected in patients using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) that confirms the presence of SARS-CoV-2 RNA. The demand for increased testing, particularly for asymptomatic individuals required alternative approaches to single-patient RT-PCR testing, such as pooling.
Methods
This study explored the impact of dilution on the detectability of SARS-CoV-2 in asymptomatic patients using RT-PCR and demonstrated that pooling can be effective in low prevalence populations.
Results
The RT-PCR results for the 3:1, 5:1, and 7:1 aliquot samples showed little differences in CT values, confirming detection capability at these dilutions.
Conclusion
Based on the results of the present study, a pooled approach with up to 5:1 sample aliquots and using the current RT-PCR methodology likely will detect SARS CoV2 RNA among asymptomatic patients.
Despite motion of the entire retinal image that results from fixational eye-movements, the visual scene is perceived as stationary. One hypothesis to account for this observation is that normal motion sensitivity is limited by the variability of fixational eye velocity. The present experiments tested this hypothesis by comparing motion sensitivity and the variability of fixational eye velocity in corresponding meridians. Speed thresholds to detect horizontal, vertical, and rotary motion in a set of eight random-dot patches were measured, while normal observers monocularly viewed the stimulus with gaze either straight-ahead or deviated to the left by 45 degrees. Eye-movement recordings using the search-coil technique were used to estimate the variability of eye velocity in the horizontal, vertical, and torsional meridians during fixation. As reported previously by Murakami (2004), the averaged thresholds for horizontal and vertical motion correlated with the averaged variability of eye velocity in the horizontal and vertical meridians when observers looked straight-ahead. However, no relationship existed between the threshold for rotary motion and the variability of eye velocity in the torsional meridian. Furthermore, no relationship existed between the motion threshold and the variability of eye velocity in any meridian during fixation in lateral eccentric gaze. These results are only partly consistent with the hypothesis that fixation variability limits motion sensitivity.
Magnitude estimation of suprathreshold stimuli provided a method of studying performance characteristics of stereoscopic depth perception across the range of functional disparities. Differences found in depth magnitude estimation as a function of the sign of disparity suggest that the neural mechanisms underlying depth perception from uncrossed disparity are more sensitive to image decorrelation, particularly at low contrast, than the mechanisms underlying depth estimation from crossed disparity. These results could occur from differences in near and far disparity-sensitive neurons, from the geometrical relationship between disparity and physical distance in normal viewing, or from the response measure independent of perception.
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