A new method has been established for the simultaneous estimation of Telmisartan and Atorvastatin calcium by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Telmisartan and Atorvastatin calcium by using boston ODS C18 column, flow rate was 1.0ml/min, mobile Phase consists of methanol:Acetonitrile:buffer in ratio of 35:25:40. Detection wave length was 235nm.The instrument used was SHIMADZU HPLC auto sampler. The retention time of Atorvastatin calcium and Telmisartan was found to be 2.350 and 3.490 minutes respectively. The analytical method was validated according to ICH guidelines (ICH Q2b). The correlation coefficient (r 2 ) was found to be 0.997 and 0.999 for Telmisartan and Atorvastatin calcium respectively. % mean recovery was found to be 100.943% and 100.576% for Telmisartan and Atorvastatin calcium respectively. %RSD for precision on replicate injection was 0.46 and 0.70 for Telmisartan and Atorvastatin calcium respectively. The validation study was found to be precise, robust, and repeatable.
Different dosage forms namely tablets, capsules, creams and syrups were analysed for curcumin content, by the well-known spectrophotometric method. Turmeric extract powder was used as a source of curcumin in capsule and tablet formulations. Turmeric oleoresin was used as a source of curcumin in cream formulation. Additionally, syrup formulations containing turmeric extract powder as well as turmeric oleoresin, separately, were also tested for their curcumin contents. Analytical results for curcumin content were found to be satisfactory in tablets and capsules containing turmeric extract powder. Whereas, in case of syrup containing turmeric extract powder, analytical findings for curcumin content, did not meet the expected specifications. However, when turmeric powder was replaced by the same quantity of oleoresin having similar strength, in cream and syrup, the results met the expected values, at the initial stages. But analysis data over a period of 1Year testing, showed declination in the initial findings due to unstability of turmeric oleoresin for long duration
The traditional drug, as characterized by the World Health Organization, is the aggregate of the information, aptitudes, and practices dependent on the hypotheses, convictions, and encounters indigenous to various societies, regardless of whether intelligible or not, utilized in the upkeep of well-being just as in the anticipation, analysis, improvement, or treatment of physical and psychological maladjustment. There is increasing usage of traditional drugs worldwide. To adequately manage safety issues associated with traditional drugs, the future dentists must possess good knowledge of them. Dental undergraduate students, totaling 100 students completed a questionnaire in a cross-sectional study that assessed their knowledge and attitude towards traditional drugs. Data was analyzed using SPSS software. The best known and used herb was chamomile and clove. Although with limited knowledge, the dental students showed a high level of personal use and good attitudes towards traditional drugs. Introduction of traditional drugs courses in their dental curriculum and also more awareness programs should increase their knowledge and attitude, so they could in the future adequately manage patients who used or intend to use traditional drugs.
Objective: The main objective of the present work is to develop an efficient, unique, reliable Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method for the simultaneous quantification of Amoxicillin (AMX), Clarithromycin (CTM) and Lansoprazole (LPZ) in bulk and pharmaceutical formulations. Methods: The chromatographic separation was achieved by using Kinetex column C18 (100 x 4.6 mm, 2.6 µm) with Buffer (2.5 g of hexane sulphonic acid and 1ml of Triethylamine which are added to 1000 ml of HPLC water and adjusted its pH at 5.0 with Ortho phosphoric acid) and acetonitrile in the ratio of 70: 30 (%v/v) as a mobile phase at flow rate of 1.0 ml/min. The column effluents were monitored by a photodiode array detector at wavelength predetermined at 240 nm. Results: The method produced reliable results at optimized chromatographic conditions. The method was linear at concentration range of 15-225 µg/ml of AMX, 15-225 µg/ml of CTM and 0.9-13.5 µg/ml of LPZ with regression coefficients of 0.9999, 0.9999, and 0.9999 respectively. The retention times of AMX, CTM, LPZ were obtained as 1.513, 3.124, 3.770 min respectively. Results obtained for system suitability, precision, LOD and LOQ were in acceptable range and were validated according to the guidelines of the International Council for Harmonization (ICH). Conclusion: The proposed method was validated in accordance with ICH and all the obtained results were found satisfactory and were successfully applicable to the analysis of the bulk and the pharmaceutical formulations.
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