AuthorshipMK participated in the conception and design of the study and coordinated the data collection. TA critically reviewed the proposal, did the data analysis, the write up and prepared the manuscript for publication. BG and AA contributed by critically reviewing the proposal, design of the study, and data analysis. All authors read and approved the final manuscript.
The article contains analysis of perspectives of cell technologies use in the therapy of patients with Alzheimer's disease (AD). Currently there are no effective ways to diagnose this disease at early stage. Late diagnostics of AD and weak understanding of its mechanisms complicate clinical tactics leaving in fact palliative (symptomatic) treatment as the only available option. Authors propose a combination of the traditional methods of treatment with newly developed cell therapy technologies. Stem cells (SC) implantation is the basis for recovery of functional activity of neural networks in the regions affected by neurodegeneration. Based upon conducted experimental studies and analysis of scientific literature, the authors recommend to use the technique of the intranasal perineural application of autologous mesenchymal stem cells (MSC) for the treatment of patients with Alzheimer's disease. Following the administration into the submucosa of nasal cavity, SC migrate along the olfactory nerve to the brain promoting a reparative effect, particularly attributed to the excretion of neurotrophic factors (brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), insulin growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF)).
Hypothesis on migration of stem cells to injured brain region after introduction of stem cells into nasolacrimal duct has been experimentally verified on Wistar rats (n=7). Fluorescent stem cells have been detected in the injured region of frontal cerebral cortex using confocal microscopy in 20 hours after implantation of mesenchymal stem cells into nasolacrimal duct of rats.
Thua Thien Hue province, a coastal province in Central Vietnam has strongly affected by climate change, especially in aquaculture production. This paper reviews the results of household survey and key informant reviewing on effect of steam climate events and natural disasters affect aquaculture in five districts of the province.
Background: Neuro destructive processes of any etiology are related to problematic and socially important diseases due to ineffective therapeutic strategy and need to search for new successful ways of treatment and rehabilitation of patient with cerebral infarctions and brain attacks Aims: Authors plant overify hypothesis on viability of additional use of perineural implantation of autologous mesenchymal stem cells (MSC) in order to optimize standard therapy of patients with brain attacks. Such combined technology is aim datextra activation of brain plasticity mechanisms during development of neuro destructive processes. Methods: The technique of MSC perineural migration to injured brain regions was experimentally verified on rats (n=40) paying attention to somatotopic organization of cranial nerves. This technique was clinically tested in pilot project. Phenotyping of autologous MSC from adipose tissue (AT) was performed in 23 patients with brain attacks. These 23 patients received standard treatment as per international guidelines together with three perineural implantations of autologous MSC from AT with 5-9days intervals. The other group of patients (n=7) received only standard therapy as per international guidelines. Results: Additional use of cell therapy resulted in more rapid and effective recovery of disordered neurological functions in all cases compared to those who received standard therapy. The phenomenon of abrupt recovery of neurological functions was established during first 24hours after each injection of autologous MSC. Cumulative recovery of functions progressed after each implantation. Discussion and conclusion: Experimentally developed technique of perineural implantation of autologous MSC was successfully verified in clinical conditions in accordance with certified cell therapy guideline (The Ministry of Health of the Republic of Belarus) in combination with standard treatment of patients with cerebral infarctions. Cell therapy with autologous MSC from AT by means of perineural delivery to injured brain regions is the basis for activation of reparative potential of nerve tissue and progressive recovery of neurological functions in patients with cerebral infarctions.
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