Essential oils from leaves and stems of Phlomis lurestanica at the flowering stage were extracted by hydrodistillation. The essential oils were analyzed using Gas Chromatography and Gas Chromatography-Mass Spectrometry. Twenty-eight and twenty-five compounds were identified in the stems and leaves, respectively. The main compounds of stems were α-pinene (12.40%), γ-cadinene (10.92%), and γ-elemene (6.46%). Hexadecane (8.97%), 2-dodecnenal (6.57%), and heptadecane (6.32%) were the major constituents in the essential oils of the leaves. The methanol extracts of stems and leaves were evaluated for their antioxidant properties using 2,2-diphenyl-1-picrylhydrazyl and β-carotene/ linoleic acid tests. In the 2,2-diphenyl-1-picrylhydrazyl assay, the leaves (IC 50 = 1168.9 µg/ml) have stronger antioxidant activity than the stems (IC 50 = 1563.6 µg/ml). The extracts of the leaves and stems showed remarkable antioxidant activity in β-carotene/linoleic acid assay (96.2% and 95%, respectively). The total phenol and flavonoid contents of the species were determined using Folin-Ciocalteu and AlCl 3 assays, respectively. The phenol and flavonoid contents of the leaves (301.0 µg/ml and 45.2 µg/ml, respectively) were observed more than the stems (172.3 µg/mg and 18.8 µg/mg, respectively).
ARTICLE HISTORY
Essential oils from the leaves, stems and flowers of Postia puberula at the flowering stage were analyzed using GC and GC/MS. The results showed that leaves are abundant of cis-3-hexenyl benzoate (10.75%), benzyl benzoate (8.16%) and caryophyllene oxide (8.12%). The main compounds of stems were benzyl benzoate (21.92%), E-nuciferol (11.58%) and dibutyl phthalate (7.08%), while major components of flowers were benzyl benzoate (9.99%), caryophyllene oxide (8.14%) and E-nuciferol (8.13%). The antioxidant activities of methanol extract were evaluated by DPPH and β-carotene/linoleic acid assays. The results showed that in both methods, leaves had stronger antioxidant activity than other organs.
This study was carried out to evaluation of solvent type on antioxidant potential and phenol and flavonoid contents of Postia puberula belonging to the Astraceae family. Ethanolic, methanolic, aqueous and hydroalcoholic extracts were prepared for evaluation of the antioxidant activities and phenol and flavonoids contents. The antioxidant effects were tested by two methods, 2 and 2 diphenyl 1picryl hydrazyl (DPPH) and beta-carotene-linoleic acid. Gallic acid and quercetin were used as controls for phenolic and flavonoid compounds, respectively. The results showed that the solvent type could be effective on antioxidant activity, phenol and flavonoid content, so that the hydroalcoholic extract had the highest antioxidant activity in both methods, also, the highest phenol and flavonoid contents were observed in the hydroalcoholic and ethanolic extracts, respectively.
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