This study was undertaken to observe the prevalence, serogroup, avian pathogenic Escherichia coli (APEC)-associated virulence gene, randomly amplified polymorphic DNA (RAPD) pattern, and antibiotic resistance genes of E. coli in backyard layers and their environment in India. From the 360 samples of healthy layers and their environment, 272 (75.5%) E. coli were isolated. The majority (28.67%) of them were untypeable. Among the studied virulence genes (papC, tsh, iucC, astA), 52 (14.32%) isolates were found to possess astA, including the isolates from the drinking water of the birds (4/272, 1.47%). These strains belonged to 18 different serogroups. Most of the isolates were typeable by RAPD and they produced different patterns. Phenotypic resistance of the isolates was most frequently observed to erythromycin (95.83%), chloramphenicol (87.52%), and cotrimoxazole (78.26%). None of the isolates was found to possess extended-spectrum beta-lactamases (bla(TEM), bla(SHV), bla(CTX-M) or quinolone resistance (qnrA) genes by PCR. The present study was the first attempt in India to assess APEC distribution in backyard poultry production.
River water quality in rapidly urbanising Asian cities threatens to damage the resource base on which human health, economic growth and poverty reduction all depend. Dhaka reflects the challenges and opportunities for balancing these dynamic and complex trade-offs which goals can be achieved through effective policy interventions. There is a serious problem of water pollution in central Dhaka, in the Turag-Tongi-Balu River system in Bangladesh with the river system being one of the most polluted in the world at the moment. A baseline survey of water chemistry and total coliforms has been undertaken and shows dissolved oxygen close to zero in the dry season, high organic loading together with extreme levels of Ammonium-N and total coliform in the water. Models have been applied to assess hydrochemical processes in the river and evaluate alternative strategies for policy and the management of the pollution issues. In particular models of flow, Nitrate-N, Ammonium-N and indicator bacteria (total coliforms) are applied to simulate water quality in the river system. Various scenarios are explored to clean up the river system, including flow augmentation and improved effluent treatment. The model results indicate that improved effluent treatment is likely to have a more significant impact on reducing Ammonium-N and total coliforms than flow augmentation, but a combined strategy would greatly reduce the pollution problems in the Turag-Tongi-Balu River System.
Mannan-binding lectin (MBL) deficiency is determined by MBL gene polymorphisms and is associated with an increased infection risk. To clarify the role of MBL in Allo-SCT, 131 recipients-donors were analysed. MBL genotypes were determined by PCR and heteroduplex analyses, MBL serum levels by ELISA, and MBL oligomers by western blotting. MBL levels o400 ng/ml were associated with increased susceptibility to fungal pneumonia (7/12 vs 35/111; P ¼ 0.04, adjusted P ¼ 0.002), HSV/VZV (7/12 vs 26/111; P ¼ 0.03), CMV reactivation and acute GVHD. Donor genotypes had no influence. Pre-SCT MBL levels corresponded to recipients' genotypes (Po0.001), changed significantly post-SCT, but were not influenced by donors' genotypes. MBL oligomer profiles were similar pre-/post-SCT. Cultured CD34 þ cells were found not to synthesise MBL. In conclusion, low MBL levels pre-transplant predisposed patients to sepsis, fungal and viral infection. Donors' MBL genotypes did not influence infection rates. Prospective studies should clarify the importance of MBL as a prelude for MBL replacement after SCT.
Abstract:The impact of cyclone track features (e.g., cyclone translation speed, cyclone path and cyclone landfall crossing angle) in combination with tidal phase shift upon surge characteristics have been investigated at the Bay of Bengal along the Bangladesh coast. A two-dimensional hydrodynamic model in a horizontal direction (2DH) coupled with a storm-surge model has been employed for the study. Numerical experiments with three different cyclone translation speeds show that when the surge height is directly forced by the cyclonic wind speed especially within the RWM (Radius of Maximum Wind), faster translation speed produces reduced surge height as the cyclone gets less time to force the water. On the other hand, at locations outside the RMW, surge waves travel as a propagating long wave where higher surges are produced by faster moving cyclones. It is found that surge arrival times are more and more affected by tidal phase when cyclone translation speed is reduced. Analysis of seven hypothetical parallel cyclone paths show that local bathymetry and complex coastline configurations strongly influence the surge height and surge arrival time along the Bangladesh coast. From the analyses of cyclone landfall crossing angles at the Khulna and Chittagong coasts, it is observed that surge durations are the smallest at both the coasts when the coastline crossing angles are the smallest.
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