One of the major concerns in photoacoustic computed tomography (PACT) is obtaining a high-quality image using the minimum number of ultrasound transducers/view angles. This issue is of importance when a cost-effective PACT system is needed. On the other hand, analytical reconstruction algorithms such as back projection (BP) and time reversal, when a limited number of view angles is used, cause artifacts in the reconstructed image. Iterative algorithms provide a higher image quality, compared to BP, due to a model used for image reconstruction. The performance of the model can be further improved using the sparsity concept. In this paper, we propose using a novel sparse dictionary to capture important features of the photoacoustic signal and eliminate the artifacts while few transducers is used. Our dictionary is an optimum combination of Wavelet Transform (WT), Discrete Cosine Transform (DCT), and Total Variation (TV). We utilize two quality assessment metrics including peak signal-to-noise ratio and edge preservation index to quantitatively evaluate the reconstructed images. The results show that the proposed method can generate high-quality images having fewer artifacts and preserved edges, when fewer view angles are used for reconstruction in PACT.
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Significance:
Photoacoustic tomography (PAT) is a widely explored imaging modality and has excellent potential for clinical applications. On the acoustic detection side, limited-view angle and limited-bandwidth are common key issues in PAT systems that result in unwanted artifacts. While analytical and simulation studies of limited-view artifacts are fairly extensive, experimental setups capable of comparing limited-view to an ideal full-view case are lacking.
Aims:
A custom ring-shaped detector array was assembled and mounted to a 6-axis robot, then rotated and translated to achieve up to
steradian view angle coverage of an imaged object.
Approach:
Minimization of negativity artifacts and phantom imaging were used to optimize the system, followed by demonstrative imaging of a star contrast phantom, a synthetic breast tumor specimen phantom, and a vascular phantom.
Results:
Optimization of the angular/rotation scans found
effective detectors were needed for high-quality images, while 15-mm steps were used to increase the field of view as required depending on the size of the imaged object. Example phantoms were clearly imaged with all discerning features visible and minimal artifacts.
Conclusions:
A near full-view closed spherical system has been developed, paving the way for future work demonstrating experimentally the significant advantages of using a full-view PAT setup.
Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.
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