Journal homepage: www.zums.ac.ir/jhehp Background: Neutrophils are the most abundant white blood cells in humans. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. NETs is a new strategy for pathogen response. This study focused on whether LPS induced NETs release in vitro in the HL60 cell line. Methods: In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. Trypan blue as vital staining was used for viability. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA. Results: Our results showed that 75% NETs was produced by HL-60 differentiated neutrophil cells exposed to 200 ng/ml LPS in 45 minues. Conclusion: Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.
Journal homepage: www.zums.ac.ir/jhehp Background: Helicobacter pylori play a significant etiological role in various digestive diseases. Peptic ulcer is caused by H. pylori, which destroys the duodenum mucus and is often observed in the individuals consuming tobacco, spicy and heavy meals, alcohol, coffee, and tranquilizers. Several studies have indicated that duodenal ulcer promoting genes dupA and cagA are involved in H. pylori etiology. The present study aimed to evaluate the correlation between these genes and peptic ulcer. Methods: In this study, 500 stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations. Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < 0.05). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection. Conclusion: According to the results, there are significant correlations between tobacco use (P < 0.05), tranquilizer use (P < 0.05), and meteorism (P < 0.05) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with nonulcer dyspepsia and peptic ulcer.
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