Graphen oxide has emerged as a promising tool in medical biotechnology due to its outstanding properties applicable in several fields as well as cell imaging, drug and gene delivery. Monolayer structure and high surface area of Graphen benefits elevated loading capacity of drugs rather than other nanomaterials. However Graphen oxide in physiological solutions has unfavourable reactions which confine it’s application in biomedical field without additional surface functionalization. Coating of graphenoxide by polyethylenimine is an approach to enhance biocompatibility of graphen oxide and also provides desirable physicochemical features for oligonucleotides delivery. The data presented here is related to graphenoxide-PEI characterisation and it’s cytotoxicity assay on variouse breast cancer cell lines including MDA-MB-468 and MDA-MB-231 and MCF7 by MTT assay.
The data provided in this article are related to research entitled “Efficiency of graphene oxide nanoparticles as delivery system of SOX2OT siRNA”. In this research, the goal is to use PEI-functionalized graphene oxide (PEI-GO) as a carrier for SOX2OTsiRNA delivery. In this article describes how GO coated with PEI and it was tested whether it can be siRNA carrier in NTERA2? Can it absorb siRNA? Whether Go-PEI affects the viability of NTERA2 (NT2: human embryonic carcinoma stem cell), and HeLa cell lines. In this experiment, graphene oxide nanoparticles functionalized with a polycationic polymer, polyethylenimine (PEI). GO-PEI formation was verified with DLS, FTIR tests and zeta sizer. siRNA absorption ability of GO-PEI was tested by gel retardation assay in various weight ratios of GOPEI/siRNA (GOPEI weight/siRNA weight) (w/w ratio). The cell lines were treated with different concentrations of GO-PEI nanoparticles for 24 and 48 hours. Also, the NT2 cells were treated with different concentrations of GO-PEI nanoparticles and PEI for 36 hours. Cytotoxicity of GO-PEI were investigated by calculating the percent of cell survival by MTT assay. MTT data analyzed in excel. Researchers, who want to research on different drugs, could transfer the drug to NT2, HeLa and other cancer cells on GO-PEI (concentration 0 up to 100 mg/L).
LINK-A (long intergenic non-coding RNA for kinase activation) is a newly identified long non-coding RNA with oncogenic function, which leads to the hyperactivation of AKT and HIF1α. thereby, fosters cell proliferation, mobility and metastasis. VEGF (vascular endothelial growth factor), a well-known cytokine has an important role in angiogenesis. In this study, we quantified RNA expression of LINK-A and VEGF on 45 tumor specimens obtained from Iranian patients diagnosed with Epithelial Ovarian Cancer (EOC). Our goal was to evaluate expression of LINK-A lncRNA and VEGF mRNA in ovarian cancer tissues and find the probable correlation of LINK-A with VEGF as a major transcription target for HIF1α. LINK-A and VEGF were remarkably overexpressed in EOC tissues compared to normal tissues (P value: 0.004, 0.0001, respectively), but we did not find correlation between LINK-A and VEGF RNA expressions in this study. LINK-A was significantly overexpressed in higher stages of cancer and tumor grades. VEGF was only significantly elevated in higher stages. This study confirms importance of novel lncRNA of LINK-A in Iranian EOC patients.
The oncogenic role of long non-coding RNA SOX2 overlapping transcript (SOX2-OT) has been demonstrated as a miRNA decay system that sponges to tumor suppressor miRNA including miR-122-3p in glioblastoma and miR-194-5p in glioblastoma, gastric and colorectal cancers. Although, the molecular function of SOX2-OT is still unknown in most cancer including lung cancer. As aim of current study, we evaluated downstream regulation function of SOX2-OT in A549 and Calu-3 lung cancer cell lines. We knock down SOX2-OT expression with using of RNA interference system that had significant decreased expression in A549 and Calu-3 cells. Then, expression of down-regulating miRNAs (miR-122-3p and miR-194-5p) evaluated that showed increased expression of miR-122-3p and miR-194-5p. Also, expression of miRNAs downstream mRNA including FOXO1 (Forkhead Box O1) and FOXA1 (Forkhead Box O1), changed. Recently, Critical roles of FOXO1 and FOXA1 proteins in pathways that involved in proliferation, metastasis and apoptosis has been demonstrated. Downstream changes in cellular traits, as evaluated by MTT, flow cytometry, metastasis and apoptosis assays proved that biological behaviors of lung cancer cells influenced after SOX2-OT knockdown. Overall, results of current study bring up oncogenic role for SOX2-OT via regulation of miR-122-3p/FOXO1 and miR-194-5p/FOXA1 axes.
Non-coding RNA elongated (lncRNAs) have recently attracted as molecules that regulate gene expression of the pluripotent properties (pluripotency) of stem cells. Recently our colleagues examined the role of one of these RNAs called SOX2OT in esophageal squamous cell carcinoma, and found a concomitant increase in its expression with some regulatory genes of cell proliferation. In the present study, using the design of suitable primers from SOX2OT gene, we investigated the effect of siRNA on expression of SOX2OT.
The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC.
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