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The aim of the work is to synthesize iron oxide (α-Fe2O3) nanoparticles using Sida cordifolia plant extract along with evaluation of its antibacterial activity. The presence of phytochemicals in Sida cordifolia methanolic plant extract was investigated by HPTLC and LC-MS/TOF. The probable mechanism for formation of α-Fe2O3 nanoparticles in mediation with plant extract was demonstrated. The green synthesized iron oxide nanoparticles (α-Fe2O3 NPs) were characterized by using X-ray diffraction, scanning, and transmission electronic microscopy, TG-DTA, FTIR, and UV spectroscopy. The crystallite size of prepared α-Fe2O3 nanoparticles estimated via Debye-Scherrer formula and Williamson-Hall plot was around 20 nm which is in accordance with particle size in TEM images. The S. cordifolia mediated iron-oxide nanoparticles (α-Fe2O3 NPs) hold potent antibacterial activity against various gram positive and gram negative bacteria.

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The present investigation aims to synthesize copper oxide nano rods using Asparagus racemosus roots extract (Ar-CuO) in an ecofriendly process. The synthesized CuO nano rods are characterized by XRD, SEM, TEM, FT-IR, Raman and UV-visible spectroscopy. X-ray diffraction pattern showed typical monoclinic structure. SEM and TEM analysis revealed the formation of self-assembled CuO nano rods with diameter and length ~ 50-100 nm and 400-500 nm respectively. The antimicrobial activity of green synthesized copper oxide nanorods was tested against various bacterial pathogens i.e., E. coli, B. subtilus, K. pneumonia, A. hydrophila, P. fluorescens, Y. ruckeri, F. branchiophilum and E. tarda with fascinating action towards harmful bacteria.