Recapitulating radioresistant
cell features in pertinent cell line
models is essential for deciphering fundamental cellular mechanisms.
The limited understanding of passage and cell cycle phases on radioresistant
cells revived post-cryopreservation led us to investigate the effect
of sub-culturing in parental and radioresistant MCF-7 cells. In this
study, the radioresistant cells showed high-intensity nucleic acid
and cytochrome bands, which are potentially a radiation-induced spectral
marker. Raman spectroscopy data showed dynamic biochemical alterations
in revived radioresistant G2/M synchronized cells at early
cell passages 1 and 3 with stabilization at a latter cell passage,
5. The study highlights the importance of cell passaging and cell
cycle phases in potentially changing the biochemical parameters during
in vitro experiments after the revival of radioresistant cells post-cryopreservation.
Background
Loco‐regional recurrences attributable to field cancerization and minimal residual cancer, remain prime causes of mortality in oral cancer (OC) subjects. The current study evaluates potential of serum Raman spectroscopy (SRS) to identify recurrence‐prone OC subjects.
Methods
Raman spectra of serum from eight healthy subjects (H) and 57 OC subjects (with‐recurrence [R], without‐recurrence [NR], and with suspicious‐lesions [S]), before (BS) and after (AS) surgical excision of tumor were recorded. OC subjects were followed‐up for 7‐years.
Results
DNA and protein alterations were observed in AS sera of all groups. 4‐, 3‐, and 2‐model multivariate analyses were used to stratify BS and AS groups. H spectra were 100% distinguishable from all other groups. AS, R and NR were distinguished with high accuracy (84%) in all models. No stratification (~50%) was observed BS.
Conclusion
SRS shows potential to identify recurrence prone subjects, post‐surgery, using serum collected as early as 1 week after surgery.
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