Recapitulating radioresistant
cell features in pertinent cell line
models is essential for deciphering fundamental cellular mechanisms.
The limited understanding of passage and cell cycle phases on radioresistant
cells revived post-cryopreservation led us to investigate the effect
of sub-culturing in parental and radioresistant MCF-7 cells. In this
study, the radioresistant cells showed high-intensity nucleic acid
and cytochrome bands, which are potentially a radiation-induced spectral
marker. Raman spectroscopy data showed dynamic biochemical alterations
in revived radioresistant G2/M synchronized cells at early
cell passages 1 and 3 with stabilization at a latter cell passage,
5. The study highlights the importance of cell passaging and cell
cycle phases in potentially changing the biochemical parameters during
in vitro experiments after the revival of radioresistant cells post-cryopreservation.
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