Two different kinds of europium(III) chelates, luminescent and nonluminescent, were prepared. The chelates were coupled to bioanalytical reagents, such as antibodies, after activations of the amino group on the chelates with thiophosgene,2,4,6-trichloro-1,3,5-triazine, or iodoacetic anhydride. The reactivities of the activated luminescent chelates in the labeling of antibodies as well as the effects of both the coupling ratio and the linkage group to the luminescence quantum yield of the antibody-bound chelate were studied in aqueous buffer solution.
Free-β hCG stability is greatly improved in DBS compared to serum storage; however PAPP-A stability is decreased in the DBS medium. Despite this DBS, screening may not necessitate such strict storage and transportation rules compared to serum screening programmes.
The synthesis of novel thiazole-containing complexing agents and their luminescence properties with Eu"' and Tb"' ions are reported. One of these terpyridine analogues was also tested as an Eu"' labelling reagent, and its luminescence properties as an antibody conjugate were studied.
The performance of the AutoDELFIA® Inhibin A assay is highly acceptable for routine laboratory use for screening Down syndrome in the second trimester of pregnancy.
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