Twenty female animals selected for study from which 60% buffaloes and 40% cattle with a mean age of 6.32 ± 0.66 years. The mean duration of illness was 20.7 ± 2.13 days and majority recently calved (45%). Mean rectal temperature, respiratory and heart rates elevated and rumen motility decreased. Ferroscopy was positive in fifteen animals. Haematological and Biochemical parameters revealed leucocytosis, neutrophilia and lymphocytopenia, decreased albumin and increased total plasma proteins, globulin, AST, ALT, serum creatinine, blood urea nitrogen and plasma fibrinogen level. Laparorumenotomy revealed adhesion of reticulum and left abdominal wall in seven, reticulum and right abdominal wall in three, reticulum and ventral abdominal wall in one and reticulum with diaphragm in four cases with non-metallic foreign bodies and frothy rumen content in five animals. Ruminitis, sloughing and haemorrhages of ruminal papillae in two cases. Complications like recurrent tympany, subcutaneous emphysema, swelling and discharge at surgical site were managed appropriately and all animals recovered. In-vitro ultrasonographic examination of diaphragm revealed that the right lower half musculo-tendinous junction of diaphragm was thinner than the left lower half musculo-tendinous junction of diaphragm.

Background:Diabetes is one of the major causes of cataract. Some drugs prescribed for the treatment of diabetes are the modulators of CYP450, which may alter the risk of cataract.Objective:To study the effect of CYP450 modulation in galactosemic cataract.Materials and Methods:Male Sprague-Dawley suckling rats were allotted to four groups (n = 6), as follows: Group 1: Normal control, Group 2: Galactose control, Group 3: CYP450 inhibitor pretreated and Group 4: CYP450 inducer pretreated. Cataract was induced in animals of all groups except group 1 by feeding them galactose (50%), 21 days after parturition. From the eighteenth day of life, CYP450 inhibitor (nifedipine; 8.1 mg/kg) and CYP450 inducer (pioglitazone; 3.8 mg/kg) were given orally to groups 3 and 4, respectively. The maturation pattern of the cataract was observed by an operating microscope, every third day. Biochemical changes in the lenses of all groups, for example, CYP450 activity expressed as µM NADPH oxidized / unit time, alterations in the levels of total proteins, soluble proteins, and reduced glutathione (GSH) following the induction of cataract, were estimated.Results:The microscopic examination of the lenses indicated that CYP450 inhibitor pre-treatment delayed (fourteenth day) the occurrence of cataract, while CYP450 inducer pretreatment demonstrated an early (ninth day) cataract as compared to galactose control rats (twelfth day). A significant decrease and increase in CYP450 activity was observed with the CYP450 inhibitor and inducer pre-treatment, respectively. There was no alteration in the GSH level, but a significant increase in total and soluble protein was found in groups 3 and 4 as compared to group 2.Conclusion:CYP450 may have a role in the initiation of cataract without any effect on the maturation pattern, as revealed by the delayed occurrence of cataract with the CYP450 inhibitor and an early onset of cataract with the CYP450 inducer.

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We performed transcriptome sequencing of canine retinal tissue by 454 GS-FLX and Ion Torrent PGM platforms. RNA-Seq analysis by CLC Genomics Workbench mapped expression of 10,360 genes. Gene ontology analysis of retinal transcriptome revealed abundance of transcripts known to be involved in vision associated processes. The de novo assembly of the sequences using CAP3 generated 29,683 contigs with mean length of 560.9 and N50 of 619 bases. Further analysis of contigs predicted 3,827 full-length cDNAs and 29,481 (99%) open reading frames (ORFs). In addition, 3,782 contigs were assigned to 316 KEGG pathways which included melanogenesis, phototransduction, and retinol metabolism with 33, 15, and 11 contigs, respectively. Among the identified microsatellites, dinucleotide repeats were 68.84%, followed by trinucleotides, tetranucleotides, pentanucleotides, and hexanucleotides in proportions of 25.76, 9.40, 2.52, and 0.96%, respectively. This study will serve as a valuable resource for understanding the biology and function of canine retina.