Altogether these results could become the basis to develop new approaches aiming to rebalance the skin microbiota, which is modified during the aging process.

BackgroundLine‐field confocal optical coherence tomography (LC‐OCT) is an imaging technique providing “optical biopsies” of the skin in real time and non‐invasively. At a center optical wavelength of 1.3 µm, this innovative technology can be applied to dermo‐cosmetic product development due to both high image resolution (~2 µm) and sufficient penetration (~0.5 mm). Nevertheless, the precise dermal area analyzed with LC‐OCT has never been identified. In this study, the objective was to compare LC‐OCT images with histological sections of the same area, in order to validate a new method for in vivo and non‐invasive quantification of superficial dermis thickness. Once validated, this standardized and quantitative method was used to assess age‐related changes of the superficial dermis.Materials and MethodsEx vivo LC‐OCT acquisitions and hematoxylin‐eosin‐safran staining were performed on a panel of four healthy Caucasian female volunteers. In vivo LC‐OCT study of skin aging was performed on a panel of 37 healthy Caucasian female divided into five different age‐groups.ResultsComparison with histological sections revealed that LC‐OCT images allow the visualization and the quantification of the superficial portion of papillary dermis. Applied to different age‐group of volunteers, LC‐OCT images show a constant decrease in this superficial dermis thickness with age.ConclusionsIn conclusion, we have introduced LC‐OCT as a novel technique for in vivo and non‐invasive evaluation of superficial dermis thickness. This approach could be used in the future to demonstrate visually and quantitatively the capacity of a dermo‐cosmetic active ingredient to renormalize the structural properties of the dermis.

The inflammatory context contributes to the morphological, functional and transcriptomic changes observed in AD skin. As a result, this compromised RE model shares some characteristics with those found in AD skin and thus can be used as a relevant tool for screening formulations and drugs for the treatment of AD.

Acne is an inflammatory skin disease of the pilosebaceous unit, involving four essential factors: hyperseborrhoea combined to a modification of sebum composition, colonization by Cutibacterium (C.) acnes, hyperkeratinization and secreted inflammation. Understanding and mimicking compromised skin is essential to further develop ap

The objective was to compare LC-OCT images with histological sections of the same area, in order to validate a new method for in vivo and non-invasive quantification of superficial dermis thickness.