Development and characterization of a 3D <i>in vitro</i> model mimicking acneic skin

Laclaverie, Marine; Rouaud-Tinguely, P.; Grimaldi, Christine; Jugé, Romain; Marchand, Laëtitia; Aymard, Elodie; Closs, B.

doi:10.1111/exd.14268

Cited by 8 publications

(8 citation statements)

References 50 publications

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“…a stratified epithelium. In this model, the NHEKs are cultured on a collagen matrix placed at an air-liquid interface, producing 8–12 layers of epidermis, that can be used to study infection and inflammation by various organisms, including C. acnes [ 105 , 114 , 121 , 124 , 125 ]. Some of these RHE models, including EpiDerm, EpiSkin and SkinEthic [ [126] , [127] , [128] ] are commercially available.…”

Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

“…Some of these RHE models, including EpiDerm, EpiSkin and SkinEthic [ [126] , [127] , [128] ] are commercially available. RHE has recently been used to study biofilm formation of C. acnes (alone and in combination with Malassezia restricta ) in a pre-clinical dandruff model [ 105 ] as well as to study the interaction between acneic skin and different phylotypes of C. acnes [ 125 ].…”

Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

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The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Coenye

Spittaels

Achermann

2022

Biofilm

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Cutibacterium acnes (previously known as Propionibacterium acnes ) is frequently found on lipid-rich parts of the human skin. While C. acnes is most known for its role in the development and progression of the skin disease acne, it is also involved in many other types of infections, often involving implanted medical devices. C. acnes readily forms biofilms in vitro and there is growing evidence that biofilm formation by this Gram-positive, facultative anaerobic micro-organism plays an important role in vivo and is also involved in treatment failure. In this brief review we present an overview on what is known about C. acnes biofilms (including their role in pathogenesis and reduced susceptibility to antibiotics), discuss model systems that can be used to study these biofilms in vitro and in vivo and give an overview of interspecies interactions occurring in polymicrobial communities containing C. acnes .

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Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Coenye

Spittaels

Achermann

2022

Biofilm

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“…We here present a technical advance for the topical bacterial inoculation of a 3D human epidermal equivalent (HEE) with a minimal risk of basolateral infections, whilst allowing in vitro studies on infectious virulent strains. This methodology using glass cylinders will be easily transferable to a wide variety of advanced organotypic skin [ 62 , 63 ] or mucosal models [ 64 ], would be amenable for the application of diverse microbiota (bacteria [ 65 , 66 ], viruses [ 67 – 69 ] and fungi [ 70 , 71 ]) and can be used in every cell culture facility considering the various sizes and commercial availability, at low costs. We were able to increase the assay throughput by the large bacterial exposure area and thus obtaining multiple samples for various endpoint analysis from one single HEE.…”

Section: Discussionmentioning

confidence: 99%

Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

Rikken,

Meesters,

Jansen

et al. 2023

Microbiome

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Background Following descriptive studies on skin microbiota in health and disease, mechanistic studies on the interplay between skin and microbes are on the rise, for which experimental models are in great demand. Here, we present a novel methodology for microbial colonization of organotypic skin and analysis thereof. Results An inoculation device ensured a standardized application area on the stratum corneum and a homogenous distribution of bacteria, while preventing infection of the basolateral culture medium even during prolonged culture periods for up to 2 weeks at a specific culture temperature and humidity. Hereby, host-microbe interactions and antibiotic interventions could be studied, revealing diverse host responses to various skin-related bacteria and pathogens. Conclusions Our methodology is easily transferable to a wide variety of organotypic skin or mucosal models and different microbes at every cell culture facility at low costs. We envision that this study will kick-start skin microbiome studies using human organotypic skin cultures, providing a powerful alternative to experimental animal models in pre-clinical research.

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“…Thus, the human skin commensal Staphylococcus epidermidis , Cutibacterium acnes , and Malassezia furfur , and the transient pathogenic Staphylococcus aureus , grown over keratinocytes seeded on fibroblast-embedded fibrin matrix dermis in a Transwell ® insert, show that oil exceeding physiological levels leads to development of C. acnes or M. furfur , responsible for inflammatory acne or seborrheic dermatitis and pityriasis versicolor, respectively [ 62 ]. Moreover, the imbalance of C. acnes and peroxidized squalene treatment in a 3D model with a single epidermal layer is able to reproduce acne-prone skin, with an increase in inflammatory factors, decrease in claudin-1, and reduced epidermis integrity [ 63 , 64 ]. Although these models are a good initial approach to study cutaneous microbiome and pathologies, the addition of other microorganisms from the skin and sebaceous glands, flow, or other mechanical requirements for the model would more closely represent human skin, allowing permeability studies or simulating different pathologies caused by alterations in the microbiota.…”

Section: Biological Requirements For Skin-on-chip (Soc) Devicesmentioning

confidence: 99%

Modeling an Optimal 3D Skin-on-Chip within Microfluidic Devices for Pharmacological Studies

et al. 2022

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Preclinical research remains hampered by an inadequate representation of human tissue environments which results in inaccurate predictions of a drug candidate’s effects and target’s suitability. While human 2D and 3D cell cultures and organoids have been extensively improved to mimic the precise structure and function of human tissues, major challenges persist since only few of these models adequately represent the complexity of human tissues. The development of skin-on-chip technology has allowed the transition from static 3D cultures to dynamic 3D cultures resembling human physiology. The integration of vasculature, immune system, or the resident microbiome in the next generation of SoC, with continuous detection of changes in metabolism, would potentially overcome the current limitations, providing reliable and robust results and mimicking the complex human skin. This review aims to provide an overview of the biological skin constituents and mechanical requirements that should be incorporated in a human skin-on-chip, permitting pharmacological, toxicological, and cosmetic tests closer to reality.

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Development and characterization of a 3D in vitro model mimicking acneic skin

Cited by 8 publications

References 50 publications

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

Modeling an Optimal 3D Skin-on-Chip within Microfluidic Devices for Pharmacological Studies

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