The objective of this study was focussed on phytochemical screening, in vitro antioxidant and antiprotease activities of various solvent extracts of Muehlenbeckia platyclada root. The roots were washed thoroughly, shade dried and coarsely powdered. The powdered material of Muehlenbeckia platyclada was successively extracted with hexane, chloroform and methanol using soxhlet apparatus. Preliminary phytochemical screening for carbohydrates, proteins, alkaloids, phytosteroids, flavonoids, glycosides, polyphenolics, saponins, and tannins were done by following standard procedure. In vitro antioxidant activities of three solvent extracts were assessed using DPPH, ABTS and total antioxidant capacity and flavonoids were estimated using aluminum chloride colourimetric assay. In vitro anti-protease activity of the root was evaluated using trypsin as enzyme and BAEE (N-benzoyl-Larginine ethyl ester) as a substrate. The results showed that phytochemicals such as carbohydrates, proteins, flavonoids and glycosides, which present in the methanolic extract, were absent in hexane and chloroform extract of the root. The in vitro antioxidant and antiprotease activities of the Muehlenbeckia platyclada root clearly showed that the plant root has prominent antioxidant and protease inhibiting properties. From this work, it can be concluded that Muehlenbeckia platyclada root has the potential to be a powerful antioxidant and protease inhibitor.
Mitracarpus villosus (Sw) is a herb from Rubiaceae family used in traditional medicine for the treatment of several diseases such as hepatic, skin and venereal diseases, diarrhea and dysentery. It has also been used to treat ringworm and eczema, fresh cuts, wounds and ulcer. The aerial parts of this plant have also been used to make lotion and skin ointment used for skin diseases and infections. In this study we can evaluate the In vitro antioxidant and antiprotease activity of the methanolic extracts from Mitracarpus villosus (Sw). The methods used for antioxidant potentials include DPPH, ABTS, Nitric oxide and Hydroxy radical scavenging assays. We can evaluate the anti-inflammatory potentials by anti-protease activity. Antioxidant assays were done by using the concentrations in the range 20 -100μg/ml and the results were compared with the standard ascorbic acid. The anti-protease activity was done using BAEE (N-benzoyl-L-arginine ethyl ester) as substrate and Phenyl methyl sulphonyl fluoride (PMSF), is used as standard. The methanolic extract of the Mitracarpus villosus (Sw) showed better activity for both antioxidant and anti protease assays.
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