Young coconut juice (YCJ) from aromatic green dwarf variety fruit of Cocos nucifera L. (Family Arecaceae) is high valuated because of its nutritional and pharmacological properties (wound healing, anti-Alzheimer, anti aging). Due to the storage and handling problems, YCJ has been prepared in dry form by freeze-drying process in this study. The % yield of freeze-dried YCJ powder was 6.62 ± 0.28 %. Both of them (fresh and freeze-dried YCJ) were further studied on phytochemical, physical, chemical and microbiological properties. The results showed that β-sitosterol was isolated and identified by means of spectral data and their physical properties. The HPLC-parameters (linearity, repeatability, accuracy and specificity) were evaluated. The concentrations of β-sitosterol in HPLC analysis of both fresh and freeze-dried YCJ were 89.67 ± 0.01 and 31.84 ± 1.56 μg/ml, respectively. Furthermore, there was no significant (p>0.05) difference in physicochemical analysis in between both products. Therefore, the microbiological analysis of these products was within standard range of Thai community products standard (TCPS). In conclusion, Freeze-dried YCJ was observed to be the suitable technique to obtain the dry extract of young coconut juice.
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