Phytochemical Study and its Quantity and Quality of Fresh and Freeze-Dried Young Coconut Juice (<i>Cocos nucifera</i> L.)

Abstract

Young coconut juice (YCJ) from aromatic green dwarf variety fruit of Cocos nucifera L. (Family Arecaceae) is high valuated because of its nutritional and pharmacological properties (wound healing, anti-Alzheimer, anti aging). Due to the storage and handling problems, YCJ has been prepared in dry form by freeze-drying process in this study. The % yield of freeze-dried YCJ powder was 6.62 ± 0.28 %. Both of them (fresh and freeze-dried YCJ) were further studied on phytochemical, physical, chemical and microbiological properties. The results showed that β-sitosterol was isolated and identified by means of spectral data and their physical properties. The HPLC-parameters (linearity, repeatability, accuracy and specificity) were evaluated. The concentrations of β-sitosterol in HPLC analysis of both fresh and freeze-dried YCJ were 89.67 ± 0.01 and 31.84 ± 1.56 μg/ml, respectively. Furthermore, there was no significant (p&gt;0.05) difference in physicochemical analysis in between both products. Therefore, the microbiological analysis of these products was within standard range of Thai community products standard (TCPS). In conclusion, Freeze-dried YCJ was observed to be the suitable technique to obtain the dry extract of young coconut juice.

Physicochemical Properties of Spray-Dried Young Coconut Juice

“…After extraction, a β-sitosterol was isolated, identified and confirmed by comparison of their physical properties (TLC, HPLC, melting point (MP =135-136°C)) and spectral data with those reported in the literature [5] [14].…”

“…A total of 200 fractions (fraction size 20 ml) were collected and combined on the basis of the TLC profiles after detection with UV lamp and 50% sulfuric acid in EtOH spraying reagent. The isolated compound, β-sitosterol, was collected and identifies from previous study [5].…”

“…4) HPLC analysis HPLC analysis was performing follow as previous study [5]. The chromatographic condition was using Agilent 1100 series equipped with photodiode-array detector (PDA) and auto sampler (Palo Alto, CA).…”

“…Then, the pooled CH 2 CL 2 extract was evaporated under reduced pressure to give dried crude CH 2 CL 2 extract. This extracts are kept in well-closed containers protected from light before used [5].…”

“…YCJ has been prepared in dry form because of the storage and handling problems. Drying procession can be product manufacture of YCJ powder, for example freeze drying YCJ powder [5].One of the most commonly drying process is spray drying, widely used in commercial production of milk powders, vegetable and fruits [6], [7]. Maltodextrin (MD), encapsulating agent, was use for encapsulate YCJ powder.…”