One hundred thirty New Zealand White x California rabbits were used to study the influence of the source of dietary fiber on several digestive criteria. Five diets were formulated to provide 10% crude fiber (CF) on a DM basis. Sixty percent of this dietary fiber was supplied by alfalfa hay, citrus pulp, beet pulp, grape marc, or rice hulls in the diets. Weights of cecal contents, cecal ammonia concentration, and molar proportion of acetic acid in diets including pulps were higher and cecal levels of DM, CP, and molar proportion of butyric acid were lower than those of the alfalfa diet. Cecal ammonia, VFA, and CF concentrations of the grape marc diet were the lowest of all the diets studied; cecal CF level and molar proportion of acetic acid were significantly higher and CP level and molar proportion of butyric acid were lower in rice hull than in alfalfa diets. Diet had no influence on daily soft feces excretion (10 g DM/d), but N contribution of soft feces to N intake was higher in the diet with citrus pulp (18.7%) than in the other diets (12%). Mean retention time (R) was measured using fuchsin-stained feed and was found to be higher (21.3 h) in the rice hull diet and lower (9.3 h) in the grape marc diet than in the other diets. When coprophagy was prevented, R decreased by 0 to 7 h. In conclusion, the use of byproducts to substitute for traditional sources of fiber in rabbit diets influenced the retention time of the digesta in some segments of the gut and thus altered several digestive criteria.(ABSTRACT TRUNCATED AT 250 WORDS)

The effect of barley storage time and dietary enzyme addition on the energy value of barley-based broiler diets was studied in two experiments. A two-rowed winter barley (Beka cultivar) was stored at room temperature for 0, 3, 6, 16, and 32 wk after harvesting. At these dates, diets were formulated using 50% barley with and without the addition of a commercial beta-glucanase-based enzyme product. In Experiment 1, 320 Arbor Acres chickens (eight replicates of three 10-d-old birds and eight replicates of one 30-d-old bird, per treatment) were fed the experimental diets to determine the AMEn following a 2 x 2 x 5 (age by enzyme by barley storage time) factorial design. At the end of the metabolism trial, viscosity of the intestinal contents was determined in 30-d-old broilers. Total beta-glucan, nonstarch polysaccharides (NSP), in vitro viscosity, and endogenous enzyme activity of barley grain decreased with increasing storage time. Dietary AMEn increased with barley storage time (from 2,755 to 2,939 kcal/kg DM, P < 0.001, for 0 and 32 wk of storage, respectively), with enzyme addition (2,861 vs 2,919 kcal/kg DM, P < 0.003), and with the age of animals (2,826 and 2,958 kcal/kg DM for 10- and 30-d-old chickens, respectively, P < 0.001). Interactions of enzyme and age by barley storage time (P < 0.02 and P < 0.001, respectively) were also detected. These data indicate that the minimum time of barley storage before its inclusion in broiler feed depends on the animals' age (more than 6 wk for 10-d-old chickens and 3 wk for 30-d-old chickens), and that the use of enzymes allowed a reduction in the time of barley storage. Digesta viscosity decreased with barley storage time (P < 0.001), and with enzyme addition (P < 0.001), an interaction of storage time by enzyme addition was shown (P < 0.007). Digesta viscosity was also negatively related to the dietary AMEn content (r = -0.68, P < 0.01). In vitro barley viscosity explained 53 and 90% of the variation in gut viscosity produced by unsupplemented and enzyme supplemented diets, respectively. In Experiment 2, the same diets as Experiment 1 and the barley grain were intubated into 120 adult roosters (Hy-Line) to determine TMEn. Dietary and barley TMEn values were not affected by barley storage time or enzyme addition (3,237 and 3,037 kcal TMEn/kg DM for diets and barley, respectively).

Wheat is a common raw material used to provide energy in broiler diets. Its apparent metabolisable energy and its influence on broiler performance varies between wheat samples. Reasons for that variability can be classified as intrinsic (variety, chemical composition) and extrinsic factors (growing conditions, storage, etc.), both of which affect nutrient digestibility and availability. However, these factors are not always considered when formulating the diets for broiler chickens. Moreover, research through the years has questioned the relation between wheat AME and animal performance. This review aims to describe factors that influence the observed variability in wheat nutritive value for broiler chickens by considering origin (variety, growing conditions and post-harvest storage), chemical composition of the grain (carbohydrates and protein) and the broiler chicken.

Two experiments were conducted to determine the differences in starch digestion rate (KDS) among wheats from different cultivars and origins and to verify if chickens would benefit from a certain digestion rate of starch. In the first experiment, 192 chickens (21 d) were assigned to 4 diets containing 55% of each wheat sample (3 cultivars, one of them from 2 origins). Starch and protein digestion were calculated from the remaining starch and protein in 4 segments of the small intestine and in excreta, using chromic oxide as a marker. Mean retention time was measured in each segment, which enabled calculations of digestion rates. In the second experiment, 2,600 chickens were assigned to 5 isoenergetic and isonitrogenous diets (with KDS from 1.80 to 2.56 h(-1)) and growth performance was determined (1 to 34 d). In 3 treatments, dietary starch was provided each by the wheat cultivars (same origin), whereas in the other 2 treatments, 25 and 50% of the wheat starch with the highest KDS was substituted by pea starch. Clostridium perfringens and Lactobacillus in the cecal chyme and glucose in the blood (glycemic index) were measured in broilers at d 19 and 25, respectively. Starch was gradually digested along the small intestine, mainly in the jejunum (48.5 and 80.4% at proximal and distal jejunum) where the largest differences among wheat samples were found. Starch digestion rate varied with origin (from 1.96 to 2.56 h(-1)) and cultivar (from 2.17 to 2.56 h(-1)). Crude protein digestion rate (average 2.21 h(-1)) was not affected by either cultivar or origin. Broiler growth and feed conversion ratio improved in a quadratic way with KDS. The maximum broiler performance was observed with KDS around 2.2 h(-1). Blood glucose response (glycemic index) was not affected by KDS; therefore, it cannot be used to predict broiler performance. In conclusion, the rate of starch digestion varies among wheats, depending on both genetic and environmental conditions of the grain, and affects broiler performance.

Three experiments were conducted to study eight barley cultivars and the effect of enzyme addition on their energy value for poultry. In Experiment 1, the AMEn of a reference barley (Beka cultivar) was calculated by increasing barley concentrations (30, 40, 50, and 60%) that replaced a high protein basal diet. In Experiment 2, eight barley cultivars (four spring and four winter cultivars) replaced the reference barley in the diet with 50% barley inclusion. Two of the winter cultivars were two-rowed and two were six-rowed cultivars. A commercial enzyme was added to these diets to study the effect of enzyme addition. Diets were consumed ad libitum by 27 and 145 21-d-old Arbor Acres broiler chicks, in Experiments 1 and 2, respectively. In Experiment 3, 66 adult roosters were used to determine the TMEn of the eight cultivars used in Experiment 2. Dietary AMEn decreased linearly (P < 0.05) with increasing barley (Beka cultivar) inclusion. Beka barley AMEn was calculated by extrapolation of the linear regression equation be equal to 2,980 kcal/kg DM. Barley energy value was influenced by cultivar (P < 0.001); the spring cultivars showed greater energy value than the winter cultivars (2,963 vs 2,852 kcal AMEn/kg DM; 3,192 vs 2,929 kcal TMEn/kg DM). Two-rowed cultivars showed higher TMEn than six-rowed winter cultivars, although no differences were found for AMEn. The correlation between AMEn and TMEn values of barley was relatively low (r = 0.69); therefore, barley TMEn cannot be extrapolated to AMEn for young chicks. Enzyme addition produced an average increase of 220 kcal/kg DM in barley AMEn (P < 0.001); there was a significant (P < 0.10) interaction between barley cultivar and enzyme supplementation. The increment of barley AMEn caused by enzyme addition was partly explained (47%) by an increase in barley viscosity. This relationship implies that enzyme supplementation significantly improves the feeding value of high as compared to low viscosity barley samples, which involved a decrease in AMEn variation among cultivars for enzyme-supplemented barley. No relationship was found between AMEn of unsupplemented barley cultivars and their chemical composition. Instead, a relationship was detected for enzyme-supplemented barley; therefore two equations were proposed for predicting the AMEn of enzyme-supplemented barley to be used directly in diet formulation.

A total of 5,000 one-day-old male broiler chickens were assigned to 8 different treatments in a 4 x 2 factorial design. Four wheat cultivars (Amiro, Guadalupe, Isengrain, and Horzal) and 2 levels (0 or 1 kg/t of feed) of an enzyme cocktail (Avizyme 1300, xylanase, 2,500 U/kg and protease, 800 U/kg) were used. Nutritionally complete mash diets contained 65 and 70% of the test wheat for the starter and grower period, respectively. Test wheats were used in diets for broilers, and growth performance and AME contents were measured. Broiler performance was measured in 4,800 broilers allocated to floor pens with 75 birds each and fed from 1 to 42 d of age. Digestibilities and AME contents of diets were measured in 200 broilers from 6 to 27 d of age individually allocated to battery cages. Chromic oxide (Cr(2)O(3)) at an inclusion rate of 0.5% in the diet was used as an indigestible marker. Apparent metabolizable energy was corrected by zero N balance to obtain AME(n). Wheat cultivar strongly influenced animal performance during the starter period (1 to 21 d of age). During the grower period (21 to 42 d of age), only BW and daily feed intake were influenced by wheat cultivar. Differences in daily feed intake were associated with differences in AME(n) intake during the starter period, but not during the grower period. Nutrient digestibility was higher with the use of enzyme. Animal performance was not affected (i.e., wheat cultivar differences were not eliminated by using enzymes). During the grower period, significant interactions were detected with regard to nutrient digestibility and AME(n). Differences in AME(n) content of wheat could not be explained by digestible starch.

. 1997. Effect of dietary lard on performance, fatty acid composition and susceptibility to lipid peroxidation in growing-finishing female and entire male pigs. Can. J. Anim. Sci. 77: 301-306. The effect of increasing the energy concentration of growing-finishing pig diets through inclusion of lard on performance, carcass and meat composition and lipid susceptibility to peroxidation was studied. A total of 720 Landrace × Large White male and female pigs (equal number of entire males and females) were used. Experimental diets were formulated to have a low (3000 kcal ME kg -1 ) or high (3200 kcal ME kg -1 ) energy level, but in all cases to have a constant lysine:ME ratio. This was achieved using a low (6.8 g kg -1 ) and a high (49.3 g kg -1 ) level of dietary lard inclusion. Gilts had lower daily gain (P < 0.0001) and worse feed conversion efficiency (P < 0.0001) than entire males. Susceptibility to oxidation was not affected by sex. Fat concentration in pig diets equilibrated in metabolizable energy and protein did not modify average gain, but reduced feed intake (P < 0.02), improved the gain:feed ratio (P < 0.0001), increased dressing percentage (P < 0.02) and produced higher carcass weight (P < 0.01). Carcass backfat and lean percentage were not affected by dietary treatment. A reduction in the proportion of (n-3) fatty acids in phospholipids (P < 0.03), a higher (n-6):(n-3) ratio (P < 0.01) and a lower susceptibility of muscle homogenates to oxidation was found in animals receiving higher levels of dietary lard (P < 0.01).Key words: Pig, boar, diet, lard, lipid oxidation Lopez-Bote, C. J., Sanz, M., Isabel, B., Perez de Ayala, P. et Flores, A. 1997. Effect of dietary lard on performance, fatty acid composition and susceptibility to lipid peroxidation in growing-finishing female and entire male pigs. Can. J. Anim. Sci. 77: 301-306. On a étudié l'effet d'augmenter la concentration énergétique des aliments pour porcs en incluant du saindoux sur la croissance, sur l'utilisation des aliments, sur la composition de la carcasse et de la viande et sur la susceptibilité à l'oxydation. On a utilisé 720 porcs Landrace x Large White mâles entiers et femelles. Les diètes avaient deux niveaux d'énergie métabolisable (3000 kcal kg -1 et 3200 kcal kg -1 ) avec une proportion constante de lysine:énergie métabolisable. Le gain moyen quotidien (GMQ) des cochettes était plus faible que le GMQ des mâles et il était de même pour l'efficácité alimentaire (P < 0.0001). Aucun effet sexe n'a été observé sur l'oxydation. La concentration énergétique de diètes n'a pas affecte le GMQ mais a réduit la consommation (P < 0.02), amélioré l'efficacité alimentaire (P < 0.0001), augmenté les rendements de carcasses (P < 0.02) et a produit des carcasses plus lourds (P < 0.01). L'épaisseur de gras dorsal et les rendements en maigres n'ont pas été affectés par les diètes. Le niveaux d'inclusion de saindoux a diminué la concentration des acides gras n-3 dans les phospholipides (P < 0.03) et a provoqué un rapport (n-6)/(n-3) plus élevé (P < 0.01) ch...

1. A study was set up to investigate the influence of wheat cultivar and wheat crop nitrogen (N) fertilisation on starch (ST) digestion rate in broiler chickens. A total of 288 broiler chickens were used in a 3 x 2 factorial design with diets based on three varieties of wheat (Apache, Caphorn and Charger), each grown at two N application rates (40 and 170 kg of N/ha). 2. Starch digestion rate was determined by measuring the remaining starch and the mean retention time (MRT) in 4 segments of the small intestine (proximal and distal jejunum and proximal and distal ileum) and in excreta, using chromic oxide as a marker. 3. Varietal differences in starch content (714-746 g starch/kg DM) were smaller than differences caused by crop N fertilisation (705-755 g starch/kg DM). Nitrogen application increased wheat crude protein (CP) content from 94 to 130 g/kg DM. 4. The majority of the ST in all diets was digested by the time the digesta reached the distal ileum (average 0.84 in the distal jejunum and 0.96 in the proximal ileum). 5. Starch digestion differed among wheat cultivars in the proximal jejunum (from 0.43 to 0.57, P < 0.001). Afterwards no differences due to wheat cultivar or N fertilisation were found. 6. Starch digestion rate varied among wheat cultivars (from 2.45 to 3.28 h(-1), P < 0.001), but did not vary with N fertilisation, whereas dietary CP digestion rate was not affected by wheat cultivar or N fertilisation level. The digestion rate of ST was faster than that of CP (average 2.78 vs. 1.53 h(-1)). 7. The current study suggests that wheat cultivars can be classified on their rate of ST digestion independently of the N fertilisation applied to the crop during growth.