The mutS gene product of Escherichia coli and SalmoneUa typhimurium is one of at least four proteins required for methyl-directed mismatch repair in these organisms. A functionaBy similar repair system in Streptococcus pneumoniae requires the hex genes. We have sequenced the S. typhimurium mutS gene, showing that it encodes a 96-kilodalton protein. Amino-terminal amino acid sequencing of purified S. typhimurium MutS protein confirmed the initial portion of the deduced amino acid sequence. The S. typhimurium MutS protein is homologous to the S. pneumoniae HexA protein, suggesting that they arose from a common ancestor before the gram-negative and gram-positive bacteria diverged. Overall, approximately 36% of the amino acids of the two proteins are identical when the sequences are optimally aligned, including regions of stronger homology which are of particular interest. One such region is close to the amino terminus. Another, located closer to the carboxy terminus, includes homology to a consensus sequence thought to be diagnostic of nucleotide-binding sites. A third one, adjacent to the second, is homologous to the consensus sequence for the helix-turn-helix motif found in many DNA-binding proteins. We found that the S. typhimurium MutS protein can substitute for the E. coli MutS protein in vitro as it can in vivo, but we have not yet been able to demonstrate a similar in vitro complementation by the S. pneumoniae HexA protein.
The gene products of the mutL and mutS loci play essential roles in the dam-directed mismatch repair in both Salmonella typhimurium LT2 and Escherichia coli K-12. Mutations in these genes result in a spontaneous mutator phenotype. We have cloned the mutL and mutS genes from S. typhimurium by generating mutL-and mutS-specific probes from an S. typhimurium mutL::TnlO and an mutS::TnlO strain and using these to screen an S. typhimurium library. Both the mutL and mutS genes from S. typhimurium were able to complement E. coli mutL and mutS strains, respectively. By a combination of TnlOOO insertion mutagenesis and the maxicell technique, the products of the mutL and mutS genes were shown to have molecular weights of 70,000 and 98,000, respectively. A (I (mutL'-lacZ+) gene fusion was constructed; no change in the expression of the fusion could be detected by treatment with DNA-damaging agents. In crude extracts, the MutS protein binds single-stranded DNA, but not double-stranded DNA, with high affinity.
The product of the uvrD gene of Salmonella typhimurium LT2 and Escherichia coli K-12 is thought to play a role in both the correction of mismatched bases and the repair of DNA damage, since insertion mutations in the uvrD gene increase the spontaneous mutation frequency and make the cells more sensitive to killing by UV irradiation. To clone the uvrD gene of S. typhimurium, we first generated a uvrD-specific probe by using DNA from an S. typhimurium uvrD421::TnS mutant. This probe was used to screen a library of S. typhimurium DNA. Bacteriophage carrying intact uvrD+ genes were subsequently identified, and the uvrD+ gene was subcloned onto a low-copy-number vector. By using a combination of TnlOOO insertion mutagenesis and the maxicell technique, the product of the uvrD gene was shown to be a 75,000-dalton protein, and the relative direction of transcription of this protein was determined. Introduction of a low-copy-number plasmid carrying the S. typhimurium uvrD+ gene into uvrD insertion mutants of either S. typhimurium or E. coli restored the spontaneous mutation frequency and degree of UV sensitivity to the levels in the corresponding uvrD+ strains. Recent studies have suggested that an important system for the maintenance of genetic fidelitions of 25 t.g/ml; rifampicin and spectinomycin were added to concentrations of 100 ,ug/ml. Tetracycline, kanamycin sulfate, rifampicin, ampicillin, and polyethylene glycol (molecular weight, 8,000) were obtained from Sigma Chemical Co., St. Louis, Mo. Spectinomycin was a gift from The Upjohn Co., Kalamazoo, Mich. DNA isolations and manipulations. The isolation of plasmid and chromosomal DNAs for cloning experiments has been described elsewhere (Elledge and Walker, in press). Plasmid DNAs for restriction endonuclease analyses were isolated by the procedure of Rambrach and Hogness (20).
The uvrD gene product apparently plays a role in the repair of UV damage, in mismatch repair, and in genetic recombination. A lower level of expression of the Salmonella typhimurium LT2 uvrD gene was observed in maxicells prepared from an Escherichia coli strain that contained a lexA+ plasmid than in maxicells prepared from an E. coli strain that lacked functional LexA protein. These results suggest that the uvrD+ gene is repressed by the LexA protein and is thus a member of the set of genes whose expression is increased by "SOS"-inducing treatments.
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