The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2

Pang, P P; Tsen, S.-D.; Lundberg, Arian; Walker, Graham C.

doi:10.1101/sqb.1984.049.01.067

Cited by 23 publications

(18 citation statements)

References 17 publications

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“…This value is in agreement with previous reports that the S. typhimurium mutS protein has an Mr of 98,000 (30,31), and the E. coli protein has an Mr of 97,000 (41), both as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis. Both in vivo (30,31) and in vitro (see below) complementation experiments have shown that the E. coli and S. typhimurium mutS genes are functionally equivalent. We identified the correct initiator methionine and confirmed the initial portion of the deduced amino acid sequence by amino-terminal amino acid sequencing of purified S. typhimurium MutS protein (Fig.…”

Section: Resultssupporting

confidence: 82%

Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae

et al. 1988

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The mutS gene product of Escherichia coli and SalmoneUa typhimurium is one of at least four proteins required for methyl-directed mismatch repair in these organisms. A functionaBy similar repair system in Streptococcus pneumoniae requires the hex genes. We have sequenced the S. typhimurium mutS gene, showing that it encodes a 96-kilodalton protein. Amino-terminal amino acid sequencing of purified S. typhimurium MutS protein confirmed the initial portion of the deduced amino acid sequence. The S. typhimurium MutS protein is homologous to the S. pneumoniae HexA protein, suggesting that they arose from a common ancestor before the gram-negative and gram-positive bacteria diverged. Overall, approximately 36% of the amino acids of the two proteins are identical when the sequences are optimally aligned, including regions of stronger homology which are of particular interest. One such region is close to the amino terminus. Another, located closer to the carboxy terminus, includes homology to a consensus sequence thought to be diagnostic of nucleotide-binding sites. A third one, adjacent to the second, is homologous to the consensus sequence for the helix-turn-helix motif found in many DNA-binding proteins. We found that the S. typhimurium MutS protein can substitute for the E. coli MutS protein in vitro as it can in vivo, but we have not yet been able to demonstrate a similar in vitro complementation by the S. pneumoniae HexA protein.

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Section: Resultssupporting

confidence: 82%

Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae

et al. 1988

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“…The mutL DNA sequence of S. typhimurium predicts that the gene encodes a protein of 618 amino acid residues with a molecular weight of 67,761, which is in close agreement with data reported here and previously that mutL from S. typhimurium has an Mr of approximately 70,000 (23,24) …”

Section: Discussionsupporting

confidence: 80%

Nucleotide sequence of the Salmonella typhimurium mutL gene required for mismatch repair: homology of MutL to HexB of Streptococcus pneumoniae and to PMS1 of the yeast Saccharomyces cerevisiae

1989

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“…108) . 2 The mutH., mutL, and mutS genes of E. coli and Salmonella typ himurium have been isolated, and in the case of the E. coli genes , overproducers have been constructed (98,100,109,110). Availability of these overproducing strains and the in vitro complementation assay for mismatch repair has permitted isolation of near homogeneous, biologically active fo rms of the E. coli mutH, mutL, and mutS proteins (50, 98; K. Welsh, P. Modrich, in preparation).…”

Section: Proteins Required Fo R Dam-directed Mismatch Repairmentioning

confidence: 99%

“…Footprinting methods have demonstrated that the purified mutS protein [subunit Mr = 97,000 (98,110)] binds to at least some mismatched base pairs (98). High(:st affinity was observed for a G-T mismatch, while the protein 2As stated in the text, DNA polymerase I is not required for repair of a mismatch by the methyl-directed system.…”

Section: Proteins Required Fo R Dam-directed Mismatch Repairmentioning

confidence: 99%