Nucleotide sequence of the Salmonella typhimurium mutL gene required for mismatch repair: homology of MutL to HexB of Streptococcus pneumoniae and to PMS1 of the yeast Saccharomyces cerevisiae

Mankovich, John A.; McIntyre, Catherine; Walker, Graham C.

doi:10.1128/jb.171.10.5325-5331.1989

Cited by 42 publications

(24 citation statements)

References 39 publications

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“…Using genetic approaches and in vivo transcript analysis, we recently showed that miaA is most likely a downstream gene in a complex operon with mutL and an unknown gene, which encodes a 47-kDa polypeptide. The mutL gene product is required for methyl-directed mismatch repair (26,27,31,35), and lesions in mutL result in a distinct mutator phenotype (14,39). We also showed that insertion mutations in miaA cause a context-dependent mutator phenotype with a spectrum that seemed to be different from the one displayed by mutL mutants (15).…”

mentioning

confidence: 73%

“…The GenBank accession number is M37459. (15,27,35). Placements of mutL and the gene encoding the 47-kDa polypeptide are approximated based on the S. typhimurium DNA sequence (27).…”

Section: Methodsmentioning

confidence: 99%

“…(15,27,35). Placements of mutL and the gene encoding the 47-kDa polypeptide are approximated based on the S. typhimurium DNA sequence (27). It is not known whether downstream ORF1 is part of the operon or whether ORFi corresponds to hflx, which is very close to miaA (4).…”

Section: Methodsmentioning

confidence: 99%

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Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations

Connolly

Winkler

1991

J Bacteriol

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Previously, we reported several unusual relationships between the 2-methylthio-N6-(A2-isopentenyl)adenosine-37 (ms2i6A-37) tRNA modification and spontaneous mutagenesis in Escherichia coli K-12 (D. M. Connolly and M. E. Winkler, J. Bacteriol. 171:3233-3246, 1989). To confirm and extend these observations, we determined the structure of miaA, which mediates the first step of ms2i6A-37 synthesis, and characterized the miaA mutator phenotype. The most likely translation start of miaA overlaps the last two codons of mutL, which encodes a protein required for methyl-directed mismatch repair. This structural arrangement confirms that miaA and mutL are in the same complex operon. The miaA gene product, A2-isopentenylpyrophosphate transferase, shows extensive homology with the yeast MODS gene product, and both enzymes contain a substrate binding site found in farnysyl pyrophosphate synthetase and a conserved putative ATP/GTP binding site. Insertions in miaA cause exclusively GC->TA transversions, which contrasts with the GC--AT and AT-*GC transitions observed in mutL mutants. To correlate the absence of the ms2i6A-37 tRNA modification directly with the mutator phenotype, we isolated a unique suppressor of a leaky miaA(ochre) mutation. The miaD suppressor mapped to 99.75 min, restored the ms2i6A-37 tRNA modification to miaA(ochre) mutants, and abolished the miaA mutator phenotype. We speculate that miaD causes a decrease in ms2i6A-37 tRNA demodification or an increase in miaA gene expression but not at the level of operon transcription. Together, these observations support the idea that the ms2i6A-37 tRNA modification acts as a physiological switch that modulates spontaneous mutation frequency and other metabolic functions.The hypermodified, hydrophobic 2-methylthio-N6-(A2-isopentenyl)adenosine (ms2i6A) modification (Fig.

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mentioning

confidence: 73%

“…The GenBank accession number is M37459. (15,27,35). Placements of mutL and the gene encoding the 47-kDa polypeptide are approximated based on the S. typhimurium DNA sequence (27).…”

Section: Methodsmentioning

confidence: 99%

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Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations

Connolly

Winkler

1991

J Bacteriol

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“…A large number of N-acetyl-muramyl-L-alanine amidases have been described in bacteria (reviewed by Shockman and Hö ltje, 1994). S. typhimurium contains two genes, mapping closely to the hemF and mutL genes, which have an extensive homology to the amiA and amiB genes of E. coli which encode for N-acetylmuramyl-L-alanine amidases (Mankovich et al, 1989;Xu and Elliott, 1993 al., Xu and Elliott, 1993;Troup et al, 1994;Tsui et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Peptidoglycan structure of Salmonella typhimurium growing within cultured mammalian cells

Zöllner

et al. 1997

Molecular Microbiology

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SummaryThe cell wall structure of Salmonella typhimurium has been studied for the first time during transit from freeliving to parasitic lifestyles. Peptidoglycan of S. typhimurium proliferating within human epithelial cells contains a high proportion of previously unidentified muropeptides (5-10-fold higher than in extracellular bacteria). Amino acid and mass-spectrometry analyses showed that these new components consist of

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“…Similarly, the genes required for mismatch repair, hexA of S. pneumoniae and mutS of E. coli and Salmonella typhimurium, express homologous proteins with an overall sequence identity of approximately 36% (10,20), whereas the predicted sequences of the products of hexB in S. pneumoniae, mutL in S. typhimurium, and PMS1 in Saccharomyces cerevisiae show weak general homology (14,17,21). The sequences of UvrA from E. coli and its homolog in M. luteus exhibit less conservation than was observed for UvrB (28).…”

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confidence: 96%

Structure of the gene complementing uvr-402 in Streptococcus pneumoniae: homology with Escherichia coli uvrB and the homologous gene in Micrococcus luteus

1992

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The repair ability for UV-induced damage observed for Streptococcus pneumoniae proceeds through a system similar to the Uvr-dependent system in Escherichia coli. The DNA sequence of a gene complementing uvr-402, a mutation conferring UV sensitivity, was determined. Alignments of the deduced amino acid sequence revealed an extensive sequence homology of 55% with the UvrB protein of E. coli and 59%o with the UvrB-homologous protein of Micrococcus luteus. Nucleotide-binding site consensus was observed. The high conservation of the uvrB-like gene among these three species suggests that the role of the UvrB protein and excision repair in general might be very important for cell survival.

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Nucleotide sequence of the Salmonella typhimurium mutL gene required for mismatch repair: homology of MutL to HexB of Streptococcus pneumoniae and to PMS1 of the yeast Saccharomyces cerevisiae

Cited by 42 publications

References 39 publications

Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations

Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations

Peptidoglycan structure of Salmonella typhimurium growing within cultured mammalian cells

Structure of the gene complementing uvr-402 in Streptococcus pneumoniae: homology with Escherichia coli uvrB and the homologous gene in Micrococcus luteus

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