Zendulková D., A. Madanat, P. Lány, K. Rosenbergová, Z. Pospí‰i,: Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds. Acta Vet Brno 0000, 00: 71-77The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan.
Summary
The effects of administration of an established, inactivated IBR vaccine were studied in 30 cows from two herds (one seropositive and one seronegative). All acquired immunity which, after subsequent intratracheal infection with IBR virus, prevented development of symptoms in the cows and protected their foetuses against viral infection in utero. The calves were all healthy and were born at normal term. Ten non‐vaccinated cows from the seronegative herd responded to the experimental infection with mild respiratory disease and abortion of four out of 10 foetuses. Organs from the aborted foetuses were found to have IBR virus.
In a breeding herd, without clinical signs of disease but with 40 % of cows tested as seropositive, a 2‐year disease‐control programme was initiated. A total of 234 newborn calves were examined and it was shown that immunization of their dams with an inactivated vaccine conferred full in utero protection against IBR‐virus infection. When such calves are reared in isolation they can be used as the nucleus for a seronegative breeding herd.
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