Peanut (Arachis hypogaea L.) plants showing Tospovirus-like symptoms were observed in Co´rdoba province (central Argentina). Peanut plants were collected and tested to identify the causal agent. Tospovirus-like particles were observed by electron microscopy and Groundnut ringspot virus (GRSV) was identified by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Partial nucleotide and deduced amino acid sequences of the nucleocapsid (N) gene indicated a high degree of identity with other GRSV sequences. This is the first report of the GRSV occurrence on peanuts in Argentina.www.blackwell-synergy.com
The rapid immunofilter paper assay (RIPA) was developed to detect Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), and Tomato chlorotic spot virus (TCSV) using antisera against recombinant nucleocapsid (N) proteins of each tospovirus. The two-step RIPA was sensitive enough to detect each species specifically in only 30 min. This technique is proposed as an excellent tool for routine Tospovirus diagnosis and field epidemiological studies.
En la Argentina, desde 2007 se han observado síntomas similares a virus en campos de trigo, tales como rayas cloróticas, enanismo y amarillamiento. Se tomaron muestras de plantas y hojas sintomáticas de Marcos Juárez (2007) y de Río Cuarto (2008 y 2013), ambas localidades de la provincia de Córdoba. El virus fue transmitido experimentalmente a partir de plantas de trigo sintomáticas a los cultivares Baguette 10 y BIOINTA 3005, utilizando el vector Delphacodes kuscheli Fennah (Delphacidae); los síntomas de rayas cloróticas, enanismo y amarillamiento en los cereales inoculados se reprodujeron a los 10-15 días posinoculación. La presencia de virus se confirmó por microscopía electrónica y RT-PCR usando cebadores degenerados, que amplifican una región conservada del gen de la polimerasa (L) de los rhabdovirus de plantas. La comparación de secuencias mostró 98% de identidad de nucleótidos con el Maize yellow striate virus C. Caroya (JQ715419) aislado de maíz en Argentina. Hasta el momento, este es el primer reporte de la presencia del Maize yellow striate virus en trigo en la Argentina.
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