Diagnosis of three Tospovirus species by rapid immunofilter paper assay

Abstract

The rapid immunofilter paper assay (RIPA) was developed to detect Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), and Tomato chlorotic spot virus (TCSV) using antisera against recombinant nucleocapsid (N) proteins of each tospovirus. The two-step RIPA was sensitive enough to detect each species specifically in only 30 min. This technique is proposed as an excellent tool for routine Tospovirus diagnosis and field epidemiological studies.

La Peste Negra Del Tomate Y Morrón: Aportes Experimentales Para Su Manejo Integrado

“…También se encuentran disponibles para estos virus kits para realizar una técnica llamada RIPA, "rapid immunofilter paper assay", de muy de fácil ejecución, que brinda resultados rápidos (Tsuda et al, 1992;López Lambertini et al, 2003). Una de ellas es comercializada con el nombre de ImmunoStrip® (AGDIA, Indiana, EE.…”