Sodium dodecyl sulfate (SDS) was used in the serodiagnosis of barley double-diffusion technique in which SDS replaced Leonil SA as the viral stripe mosaic virus (BSMV) in embryos and leaves. Antisera elicited by degrading agent. Filter paper disks served as seroreactant depots. The SDS-SDS-treated preparations of partially purified BSMV were produced in disk test worked equally well with freshly prepared or lyophilized rabbits and then used in an agar gel immunodiffusion medium amended seroreactants. More than 50,000 embryos were assayed successfully. The with SDS. The serodiagnostic test was based on a modified Ouchterlony procedure shows promise for field surveys and seed testing worldwide.
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