Use of Sodium Dodecyl Sulfate in Serodiagnosis of Barley Stripe Mosaic Virus in Embryos and Leaves

Carroll, T. W.; Gossel, P. L.; Batchelor, D. L.

doi:10.1094/phyto-69-12

Cited by 19 publications

(5 citation statements)

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“…Most tests involve plating seeds on culture media. Serological tests for detection of seedborne pathogens also have been developed (Carroll et al 1979). In addition, laboratory testing procedures have been developed for many seedborne pathogens (Agarwal and Sinclair 1997).…”

Section: Discussionmentioning

confidence: 99%

Seed transmission of verticillium wilt of cotton

Göre

Erdoğan²,

Altın³

et al. 2011

Phytoparasitica

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Twenty-nine cotton genotypes with varying levels of susceptibility to Verticillium dahliae were grown in infested plots at Nazilli, Aydın, in 2008Aydın, in -2009. The highest level of disease incidence was recorded in cultivars 'BA-151', 'Celia', 'Çukurova-1518', 'Flaş' and 'Maraş 92', and averaged 85-95% for all genotypes in both years. The incidence of V. dahliae in seed averaged 29.8% for cv. Çukurova-1518, 27.6% for Flaş, 24.6% for cv. BA-151, 19.0% for cv. Celia and 16.2% for Maraş 92. Two hundred seeds from each genotype were planted, two seeds per pot, in a steam-pasteurized mixture of soil, peat, and sand. Pots were placed close to each other on a greenhouse bench to obtain a thick canopy. Typical disease symptoms appeared about 12-13 weeks after sowing. Maximum disease incidence values averaged 3.3% for Celia, 4.5% for Maraş 92, 8% for BA-151, 9% for Flaş and 9.5% for Çukurova-1518.

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Section: Discussionmentioning

confidence: 99%

Seed transmission of verticillium wilt of cotton

Göre

Erdoğan²,

Altın³

et al. 2011

Phytoparasitica

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“…The percentage of detectable BSMV-infected seeds was 0.2% as compared to 5 7o detected by SDS agar gel double diffusion (CARROLL et al 1979). It should be noted however that in both cases, levels below 0.2 % and 5 % were not tested.…”

Section: Discussionmentioning

confidence: 75%

Detection of Four Seed‐borne Plant Viruses by the Enzyme‐Linked Immunosorbent Assay (ELISA)

Faris-Mukhayyish¹,

Makkouk²

1983

Journal of Phytopathology

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This study was conducted to evaluate the sensitivity of the ELISA technique in detecting four economically important viruses, namely barley stripe mosaic (BSMV), cucumber green mottle mosaic (CGMMV), bean common mosaic (BCMV), and squash mosaic (BSMV) viruses in single seeds as well as in batches of barley, cucumber, bean and squash seeds, respectively. Results indicated the suitability of the technique in detecting the above viruses in single germinated seeds or embryos. Accordingly, seed transmission rates of BSMV, CGMMV, BCMV and SqMV were found to be 67 %, 17%, 17% and 12%, respectively. In artificially contrived mixtures of infected: healthy seeds or embryos, BSMV, CGMMV, BCMV and SqMV were successfully detected at ratios of 1 : 500, 1 : 25, 1 : 10 and 1 : 10, respectively. Sensitivity of detection was increased in the ease of BSMV by using germinated rather than ground dry BSMV‐infected barly seeds; and in the case of SqMV, by using whole germinating emybryos rather than coleoptiles only. Trials on re‐using the enzyme‐γ‐globulin conjugate indicated that CGMMV conjugate used once can be re‐used with little loss in reactivity.

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“…Successful management of barley stripe mosaic virus (BSMV) following seed certification prevented yield losses in Montana (Carroll 1983) and North Dakota, USA. At both the places, the programme followed field inspection, supplemented with serological assays (Carroll et al 1979) of the foundation seed lot. In North Dakota, no specific field inspection is done, but the samples of foundation seed, having 1000 seeds in each seed lot, are assayed by ELISA.…”

Section: Seed Certification For the Management Of Seed-borne Diseasesmentioning

confidence: 99%

Seed Health: Testing and Management

Vishunavat

Prabakar

Anand

2023

Seed Science and Technology

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Healthy seeds play an important role in growing a healthy crop. Seed health testing is performed by detecting the presence or absence of insect infestation and seed-borne diseases caused by fungi, bacteria, and viruses. The most detrimental effect of seed-borne pathogens is the contamination of previously disease-free areas and the spread of new diseases. Sowing contaminated or infected seeds not only spreads pathogens but can also reduce yields significantly by 15–90%. Some of the major seed-borne diseases affecting yield in cereals, oilseeds, legumes, and vegetables, particularly in the warm and humid conditions prevailing in the tropical and sub-tropical regions, are blast and brown spot of rice, white tip nematode and ear-cockle in wheat, bacterial leaf blight of rice, downy mildews, smuts, head mould, seedling rots, anthracnose, halo blight, and a number of viral diseases. Hence, detection of seed-borne pathogens, such as fungi (anthracnose, bunt, smut, galls, fungal blights), bacteria (bacterial blights, fruit rots, cankers), viruses (crinkle, mottle, mosaic), and nematodes (galls and white tip), which transmit through infected seed to the main crop, is an important step in the management strategies for seed-borne diseases. Thus, seed health testing forms an essential part of seed certification, phytosanitary certification, and quarantine programmes at national and international levels. Detection of seed-borne/transmitted pathogens is also vital in ensuring the health of the basic stock used for seed production and in maintaining the plant germplasm for future research and product development. Besides the precise and reproducible testing methods, appropriate practices during seed production and post-harvest handling, including seed treatment and storage, are important components of seed health management and sustainable crop protection.

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Use of Sodium Dodecyl Sulfate in Serodiagnosis of Barley Stripe Mosaic Virus in Embryos and Leaves

Cited by 19 publications

References 0 publications

Seed transmission of verticillium wilt of cotton

Seed transmission of verticillium wilt of cotton

Detection of Four Seed‐borne Plant Viruses by the Enzyme‐Linked Immunosorbent Assay (ELISA)

Seed Health: Testing and Management

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