The nodulation and growth of cowpea plants (Vigna sinensis Endl. ex Hassk. var. Poona), grown in the CERES phytotron glasshouses, were examined. The plants were grown under six controlled temperature regimes (21, 24, 27, 30, 33, or 36°C day temperature) with ammonium nitrate (NH4N03) additions of 0, 10, 30, and 90 mg nitrogen per pot of six plants; and were grown either under natural daylight or under natural daylight reduced in intensity by one-third by mesh screens. Primary root nodulation was significantly affected by temperature, NH4NO3 level, and light intensity, with an optimum temperature of 24°C. Secondary root nodulation was also affected by light intensity and temperature, but the nodulation pattern (with an optimum temperature of 33°) was almost the inverse of the primary root pattern. Temperature significantly influenced the fresh weight of nodules per plant and the nodule size, with a lesser effect of the NH4NO3 level. Plant dry weight production (tops and roots) was governed by the temperature, NH4NO3 level, and light intensity, the maximum total dry weight being produced at 27°C. Temperature, light intensity, and NH4NO3 level all influenced the plant combined- nitrogen uptake. The nodule leghaemoglobin concentration was significantly reduced at the two extreme temperatures 21° and 36°C. Temperature also markedly affected the nodule structure – particularly the distribution of starch within the nodule.

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The technique of delayed inoculation with effective, ineffective, or mixtures of effective + ineffective nodule bacteria was used to study seedling symbiosis of barrel medic, M. tribuloides, in sand culture.