Summary: A simple photometric procedure was developed for the determination of thiocyanate (SCN~) in plasma and saliva without deproteinisation or dialysis. Fe(III) ions form a red coloured complex with SCNw ith a inaximum absorbance at 460 nm. Mercury(II) nitrate is used to run a sample blank. A manual and an automated Version (COBAS ΒΙΟ) of the method is described. The method is linear up to 5000 μηιοΐ/ΐ SCN~. The CV of the between^run precision is 2.8 -8% for the manual and 2.6 -6.6% for the automated method. The SCN~ plasma concentration was 21 -134 μιηοΐ/l in nonsmokers and 44-260 μιηοΐ/ΐ in smokers. In mixed saliva, the concentration is much higher than in plasma: 1.57-5.5 mmol/1 in smokers and 0.79 -3.9 mmol/1 in nonsmokers. Plasma* but not oral fluid SCN~, is a valuable parameter for studying smoking habits in population surveys. Mowever, its use is limited. In our experience only heavy smokers can be distinguished from nonsmokers.
We evaluated the new Greiner G-400 selective multichannel analyzer. Thirty different tests can be run in combination profile and single-test mode at a rate between 150 and 300 tests per hour. The new photometer allows kinetic and equilibrium (endpoint) measurements. The G-400 analyzer demonstrated excellent precision, linearity, accuracy, ease of operation, and no carryover. Results for 16 different analytes as determined with the Hitachi 705, GSA II, and Corning 940 correlated favorably with those obtained with the G-400. The G-400 analyzer is suitable for both emergency and routine use.
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