A complement fixation test (CFT) based on that used for brucellosis (Brinley Morgan and others 1971) has been developed for use on the sera of horses exposed to the contagious equine metritis (CEM) organism. None of 50 single samples from horses thought to be unexposed to the CEM organism was positive to the test, although five showed inconclusive reactions. Samples were examined from 41 mares either proved to be infected or from an infected stud. Of these 21 were positive, 11 were inconclusive and nine were negative. The relationship of the CFT to reactions in the other tests used in this condition is discussed--the serum agglutination test (SAT) and antiglobulin test (AGT) (Benson and others 1978). Complement fixing antibodies appear to remain for a longer period in the infected animal, and are therefore more likely to be of use in the diagnosis of the chronic carrier state.
SUMMARY Serum agglutination tests, anti‐globulin tests, and complement fixation tests were carried out on sera taken over a period of 98 days from two fillies experimentally infected with the contagious equine metritis organism. The pattern, and significance in diagnosis, of these results is discussed. All 3 tests showed positive titres in the acute phase of experimental disease; reactions in the complement fixation test persisted longest. RÉSUMÉ Des tests de sero‐agglutination, anti‐globulines, et de fixation du complément ont été effectués sur des serums prélevés durant une période de 98jours sur deux pouliches infectées expérimentalement. Les résultats et leur signification diagnostique sont discutés. Les trois tests ont donné des résultats positifs durant la phase aigue de la maladie expérimentale. La réaction de fixation du complément a persisté plus longtemps. ZUSAMMENFASSUNG Serum‐Agglutinationstests, Anti‐Globulin‐Tests und Komplementbindungsreaktion wurden an Serumproben durchgeführt, die während 98 Tagen nach experimenteller Infektion mit dem CEM‐Organismus bei zwei Ponystuten gewonnen wurden. Die Signifikanz der Resultate für die Diagnose wird diskutiert. Alle drei Tests zeigten positive Titer in der akuten Phase der experimentellen Erkrankung; die KBR‐Titer persistierten am längsten.
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