The phytochemical investigation of both chloroform and ethyl acetate extracts of Centaurea microcarpa Coss. & Dur. led to the isolation of a new cyanogenicglucoside 6'-methacrylate prunasin (3) together with seven known compounds: hydroxy-11β,13-dihydro onopordaldehyde (1), β-sitosterol (2), daucosterol (4), nepetin (5), prunasin (6), astragalin (7) and 7-O-β-D-glucopyranosyl centaureidin (8). Their structures were established by spectral analysis, mainly UV, IR, ESI-MS, 1D & 2D-NMR experiments (COSY, HSQC, HMBC and ROESY).
The genus Centaurea contains more than 700 species. It is represented by 45 in Algeria. As part of a systematic examination of the Algerian species, we have investigated C. africana, which is endemic to Algeria. This work concerns the phytochemical study of the ethyl acetate soluble part of the aqueous ethanol extract of the leaves and flowers.Centaurea africana (Asteraceae) was collected during the flowering phase in May 2001, in the East of Algeria, and was authenticated by Pr. A. Kaabeche
<p>To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and <em>n</em>-butanol (<em>n</em>-BuOH) extracts of the aerial parts of <em>Genista ulicina </em>Spach. from Algeria.<strong> </strong>The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and <em>n</em>-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc<em> </em>extract exhibited good antioxidant activities comparing to the <em>n</em>-BuOH extract. Thus <em>Genista ulicina</em> could be indicated as a plant of phytopharmaceutical importance.<strong></strong></p>
Background:
The aim of the present study is to evaluate the protective effect of n-BuOH fraction of Genista vepres Pomel and Vitamin E against Isoniazid and Rifampicin (INH-RIF)-induced liver injury. Methods: Male Wistar Albino rats were dividing into eight equal groups treated with plant fraction (50 mg/kg, 100 mg/kg), vitamin E (100 mg/kg) and INH-RIF (100 mg/kg body weight /day each). At the end of the experiment, animals dissected and samples (blood, liver tissue) were removed and isolated for biochemical and histological studies.
Results :
Administration of INH-RIF for 21 days resulted in hepatic failure as evidenced by the elevation of biochemical parameters levels, and hepatic oxidative stress, which was associated with extensive hepatic parenchyma alteration. The pretreatment of the rat with G. vepres Pomel attenuated the increase of hepatic dysfunction markers, decreased significantly the level of malondialdehyde (MDA). Increased GSH level, GPx and catalase activities compared to INH-RIF treated group. However, the Vitamin E co-treatment decreased MDA level and increased GPx activity but did not show any effect in catalase or GSH parameters. The histopathological studies in the liver of rats also supported that both plant fraction and vitamin E markedly reduced the toxicity of INH-RIF and preserved the histoarchitecture of liver tissue.
Conclusion:
The results suggested that the n-BuOH fraction of G. vepres Pomel acts as a potent hepatoprotective agent against INH-RIF induced Hepatic dysfunction in rats.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.